A Novel Murine Anti-Lactoferrin Monoclonal Antibody Activates Human Polymorphonuclear Leukocytes through Membrane-Bound Lactoferrin and TLR4

Joint Authors

Hu, Xiao-Min
Xu, Yan-Rui
Yan, Ru
Sun, Shu-Liang
Dong, Hong-Liang
Wang, Jun
Gao, Xiao-Ming

Source

BioMed Research International

Issue

Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2015-11-15

Country of Publication

Egypt

No. of Pages

11

Main Subjects

Medicine

Abstract EN

Soluble lactoferrin (LTF) is a versatile molecule that not only regulates the iron homeostasis, but also harbors direct microbicidal and immunomodulating abilities in mammalian body fluids.

In contrast, little is known about the function of membrane-bound LTF (mbLTF), although its expression on human polymorphonuclear leukocytes (huPMNs) has been reported for decades.

Given that LTF/anti-LTF antibodies represent a potential diagnostic/prognostic biomarker and a therapeutic target in patients with immune disorders, we wished, in the present study, to generate a novel human LTF- (huLTF-) specific mAb suitable for detailed analyses on the expression and function of mbLTF as well as for deciphering the underlying mechanisms.

By using the traditional hybridoma cell fusion technology, we obtained a murine IgG1 (kappa) mAb, M-860, against huLTF.

M-860 recognizes a conformational epitope of huLTF as it binds to natural, but not denatured, huLTF in ELISA.

Moreover, M-860 detects mbLTF by FACS and captures endogenous huLTF in total cell lysates of huPMNs.

Functionally, M-860 induces the activation of huPMNs partially through TLR4 but independently of phagocytosis.

M-860 is thus a powerful tool to analyze the expression and function of human mbLTF, which will further our understanding of the roles of LTF in health and disease.

American Psychological Association (APA)

Hu, Xiao-Min& Xu, Yan-Rui& Yan, Ru& Sun, Shu-Liang& Dong, Hong-Liang& Wang, Jun…[et al.]. 2015. A Novel Murine Anti-Lactoferrin Monoclonal Antibody Activates Human Polymorphonuclear Leukocytes through Membrane-Bound Lactoferrin and TLR4. BioMed Research International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1054890

Modern Language Association (MLA)

Hu, Xiao-Min…[et al.]. A Novel Murine Anti-Lactoferrin Monoclonal Antibody Activates Human Polymorphonuclear Leukocytes through Membrane-Bound Lactoferrin and TLR4. BioMed Research International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1054890

American Medical Association (AMA)

Hu, Xiao-Min& Xu, Yan-Rui& Yan, Ru& Sun, Shu-Liang& Dong, Hong-Liang& Wang, Jun…[et al.]. A Novel Murine Anti-Lactoferrin Monoclonal Antibody Activates Human Polymorphonuclear Leukocytes through Membrane-Bound Lactoferrin and TLR4. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1054890

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1054890