Analysis of the Isomerase and Chaperone-Like Activities of an Amebic PDI (EhPDI)
Joint Authors
Mares, Rosa E.
Minchaca, Alexis Z.
Villagrana, Salvador
Meléndez-López, Samuel G.
Ramos, Marco A.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-28
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Protein disulfide isomerases (PDI) are eukaryotic oxidoreductases that catalyze the formation and rearrangement of disulfide bonds during folding of substrate proteins.
Structurally, PDI enzymes share as a common feature the presence of at least one active thioredoxin-like domain.
PDI enzymes are also involved in holding, refolding, and degradation of unfolded or misfolded proteins during stressful conditions.
The EhPDI enzyme (a 38 kDa polypeptide with two active thioredoxin-like domains) has been used as a model to gain insights into protein folding and disulfide bond formation in E.
histolytica.
Here, we performed a functional complementation assay, using a ΔdsbC mutant of E.
coli, to test whether EhPDI exhibits isomerase activity in vivo.
Our preliminary results showed that EhPDI exhibits isomerase activity; however, further mutagenic analysis revealed significant differences in the functional role of each thioredoxin-like domain.
Additional studies confirmed that EhPDI protects heat-labile enzymes against thermal inactivation, extending our knowledge about its chaperone-like activity.
The characterization of EhPDI, as an oxidative folding catalyst with chaperone-like function, represents the initial step to dissect the molecular mechanisms involved in protein folding in E.
histolytica.
American Psychological Association (APA)
Mares, Rosa E.& Minchaca, Alexis Z.& Villagrana, Salvador& Meléndez-López, Samuel G.& Ramos, Marco A.. 2015. Analysis of the Isomerase and Chaperone-Like Activities of an Amebic PDI (EhPDI). BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1054901
Modern Language Association (MLA)
Mares, Rosa E.…[et al.]. Analysis of the Isomerase and Chaperone-Like Activities of an Amebic PDI (EhPDI). BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1054901
American Medical Association (AMA)
Mares, Rosa E.& Minchaca, Alexis Z.& Villagrana, Salvador& Meléndez-López, Samuel G.& Ramos, Marco A.. Analysis of the Isomerase and Chaperone-Like Activities of an Amebic PDI (EhPDI). BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1054901
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054901