A Gas Chromatography-Mass Spectrometry Based Study on Urine Metabolomics in Rats Chronically Poisoned with Hydrogen Sulfide
Joint Authors
Hu, Lufeng
Deng, Mingjie
Zhang, Meiling
Sun, Fa
Yang, Xuezhi
Lin, Guanyang
Ma, Jianshe
Wang, Xianqin
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-14
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Gas chromatography-mass spectrometry (GS-MS) in combination with multivariate statistical analysis was applied to explore the metabolic variability in urine of chronically hydrogen sulfide- (H2S-) poisoned rats relative to control ones.
The changes in endogenous metabolites were studied by partial least squares-discriminate analysis (PLS-DA) and independent-samples t-test.
The metabolic patterns of H2S-poisoned group are separated from the control, suggesting that the metabolic profiles of H2S-poisoned rats were markedly different from the controls.
Moreover, compared to the control group, the level of alanine, d-ribose, tetradecanoic acid, L-aspartic acid, pentanedioic acid, cholesterol, acetate, and oleic acid in rat urine of the poisoning group decreased, while the level of glycine, d-mannose, arabinofuranose, and propanoic acid increased.
These metabolites are related to amino acid metabolism as well as energy and lipid metabolism in vivo.
Studying metabolomics using GC-MS allows for a comprehensive overview of the metabolism of the living body.
This technique can be employed to decipher the mechanism of chronic H2S poisoning, thus promoting the use of metabolomics in clinical toxicology.
American Psychological Association (APA)
Deng, Mingjie& Zhang, Meiling& Sun, Fa& Ma, Jianshe& Hu, Lufeng& Yang, Xuezhi…[et al.]. 2015. A Gas Chromatography-Mass Spectrometry Based Study on Urine Metabolomics in Rats Chronically Poisoned with Hydrogen Sulfide. BioMed Research International،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1054943
Modern Language Association (MLA)
Deng, Mingjie…[et al.]. A Gas Chromatography-Mass Spectrometry Based Study on Urine Metabolomics in Rats Chronically Poisoned with Hydrogen Sulfide. BioMed Research International No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1054943
American Medical Association (AMA)
Deng, Mingjie& Zhang, Meiling& Sun, Fa& Ma, Jianshe& Hu, Lufeng& Yang, Xuezhi…[et al.]. A Gas Chromatography-Mass Spectrometry Based Study on Urine Metabolomics in Rats Chronically Poisoned with Hydrogen Sulfide. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1054943
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054943