A Novel Injectable MagnesiumCalcium Sulfate Hemihydrate Composite Cement for Bone Regeneration

Abstract EN

Objective.

A novel injectable magnesium/calcium sulfate hemihydrate (Mg/CSH) composite with improved properties was reported here.

Methods.

Composition, setting time, injectability, compressive strength, and bioactivity in simulated body fluid (SBF) of the Mg/CSH composite were evaluated.

Furthermore, the cellular responses of canine bone marrow stromal cells (cBMSCs) and bone formation capacity after the implantation of Mg/CSH in tibia defects of canine were investigated.

Results.

Mg/CSH possessed a prolonged setting time and markedly improved injectability and mechanical property p<0.05.

Mg/CSH samples showed better degradability than CSH in SBF after 21 days of soaking p<0.05.

Moreover, the degrees of cell attachment, proliferation, and capability of osteogenic differentiation on the Mg/CSH specimens were higher than those on CSH, without significant cytotoxicity and with the increased proliferation index, ALP activity, and expression levels of integrin β1 and Coll I in cBMSCs p<0.05.

Mg/CSH enhanced the efficiency of new bone formation at the tibia defect area, including the significantly elevated bone mineral density, bone area fraction, and Coll I expression level p<0.05.

Conclusions.

The results implied that this new injectable bone scaffold exhibited promising prospects for bone repair and had a great potential in bone tissue engineering.