A New Double Stranded RNA Suppresses Bladder Cancer Development by Upregulating p21Waf1CIP1 Expression

Abstract EN

We have previously demonstrated that miR-1180-5p has potent ability to upregulate p21 expression by targeting promoter and inhibit bladder cancer.

This prompted us to conjecture that a candidate dsRNA (dsP21-397) with perfect complementarity to the miR-1180-5p target site of p21 promoter may also trigger p21 expression.

Transfection of dsP21-397 into T24 and EJ cells significantly activated p21 expression at 72 h and the activation presented in a time-course and dose-dependent manner.

Moreover, the p21-activated activities of dsP21-397 and miR-1180-5p are not significantly different.

Overexpression of p21 downregulated Cyclin D1, CDK4/6, and Cyclin A2 expression, and thereby induced cell cycle arrest and inhibited proliferation.

Moreover, dsP21-397 suppressed bladder cancer largely depended on manipulating p21.

In conclusion, our study identifies a pair of miRNA-dsRNA mediating endogenous p21 overexpression.