A New Double Stranded RNA Suppresses Bladder Cancer Development by Upregulating p21Waf1CIP1 Expression
Joint Authors
Wang, Chenghe
Ge, Qiangqiang
Chen, Zhong
Hu, Jia
Li, Fan
Song, Xiaodong
Xu, Hua
Ye, Zhangqun
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-30
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
We have previously demonstrated that miR-1180-5p has potent ability to upregulate p21 expression by targeting promoter and inhibit bladder cancer.
This prompted us to conjecture that a candidate dsRNA (dsP21-397) with perfect complementarity to the miR-1180-5p target site of p21 promoter may also trigger p21 expression.
Transfection of dsP21-397 into T24 and EJ cells significantly activated p21 expression at 72 h and the activation presented in a time-course and dose-dependent manner.
Moreover, the p21-activated activities of dsP21-397 and miR-1180-5p are not significantly different.
Overexpression of p21 downregulated Cyclin D1, CDK4/6, and Cyclin A2 expression, and thereby induced cell cycle arrest and inhibited proliferation.
Moreover, dsP21-397 suppressed bladder cancer largely depended on manipulating p21.
In conclusion, our study identifies a pair of miRNA-dsRNA mediating endogenous p21 overexpression.
American Psychological Association (APA)
Wang, Chenghe& Ge, Qiangqiang& Chen, Zhong& Hu, Jia& Li, Fan& Song, Xiaodong…[et al.]. 2015. A New Double Stranded RNA Suppresses Bladder Cancer Development by Upregulating p21Waf1CIP1 Expression. BioMed Research International،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1054977
Modern Language Association (MLA)
Wang, Chenghe…[et al.]. A New Double Stranded RNA Suppresses Bladder Cancer Development by Upregulating p21Waf1CIP1 Expression. BioMed Research International No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1054977
American Medical Association (AMA)
Wang, Chenghe& Ge, Qiangqiang& Chen, Zhong& Hu, Jia& Li, Fan& Song, Xiaodong…[et al.]. A New Double Stranded RNA Suppresses Bladder Cancer Development by Upregulating p21Waf1CIP1 Expression. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1054977
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054977