Three-Dimensional Modelling inside a Differential Pressure Laminar Flow Bioreactor Filled with Porous Media
Joint Authors
Weyand, Birgit
Israelowitz, Meir
Kramer, James
Bodmer, Christian
Noehre, Mariel
Schmälzlin, Elmar
Gille, Christoph
von Schroeder, Herbert P.
Strauß, Sarah
Reimers, Kerstin
Vogt, Peter M.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-02
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
A three-dimensional computational fluid dynamics- (CFD-) model based on a differential pressure laminar flow bioreactor prototype was developed to further examine performance under changing culture conditions.
Cell growth inside scaffolds was simulated by decreasing intrinsic permeability values and led to pressure build-up in the upper culture chamber.
Pressure release by an integrated bypass system allowed continuation of culture.
The specific shape of the bioreactor culture vessel supported a homogenous flow profile and mass flux at the scaffold level at various scaffold permeabilities.
Experimental data showed an increase in oxygen concentration measured inside a collagen scaffold seeded with human mesenchymal stem cells when cultured in the perfusion bioreactor after 24 h compared to static culture in a Petri dish (dynamic: 11% O2 versus static: 3% O2).
Computational fluid simulation can support design of bioreactor systems for tissue engineering application.
American Psychological Association (APA)
Weyand, Birgit& Israelowitz, Meir& Kramer, James& Bodmer, Christian& Noehre, Mariel& Strauß, Sarah…[et al.]. 2015. Three-Dimensional Modelling inside a Differential Pressure Laminar Flow Bioreactor Filled with Porous Media. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1055050
Modern Language Association (MLA)
Weyand, Birgit…[et al.]. Three-Dimensional Modelling inside a Differential Pressure Laminar Flow Bioreactor Filled with Porous Media. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1055050
American Medical Association (AMA)
Weyand, Birgit& Israelowitz, Meir& Kramer, James& Bodmer, Christian& Noehre, Mariel& Strauß, Sarah…[et al.]. Three-Dimensional Modelling inside a Differential Pressure Laminar Flow Bioreactor Filled with Porous Media. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1055050
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055050