MiR-183 Regulates ITGB1P Expression and Promotes Invasion of Endometrial Stromal Cells
Joint Authors
Chen, Jie
Gu, Lin
Ni, Jie
Hu, Ping
Hu, Kai
Shi, Ying-Li
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-19
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
We applied in the previous study miRNA microarray screening analysis to identify several differentially expressed miRNAs, including miR-183 in normal, eutopic, and ectopic endometrium.
Knockdown ofmiR-183 expression induced the invasiveness and inhibition of apoptosis in endometrial stromal cells.
The current study aims to identify the miR-183 targets with relevance to cell functions in endometrial stromal cells, to verify the interaction of miR-183 with its target genes, and to confirm the role of miR-183 in the process of endometriosis.
Using microarray analysis, we identified 27 differentially expressed genes (19 were upregulated and 8 downregulated), from which we selected 4 downregulated genes (ITGB1, AMIGO2, VAV3, and PSEN2) based on GO databases for functional analysis and significant pathway analysis.
Western blotting analyses showed that integrin β1 (ITGB1), but not AMIGO2, was affected by miR-183 overexpression, whereas no protein expression of VAV3 and PSEN2 was detected.
Luciferase reporter assay verified that ITGB1 is a target gene of miR-183.
Moreover, we found that ITGB1 is overexpressed in the endometrium of endometriosis patients.
Furthermore, overexpression of ITGB1 rescued the repressive effects of miR-183 on the invasiveness of endometrial stromal cells.
These findings, together with the fact that ITGB1 is a critical factor for cell adhesion and invasiveness, suggest that miR-183 may be involved in the development of endometriosis by regulating ITGB1 in endometrial stromal cells.
American Psychological Association (APA)
Chen, Jie& Gu, Lin& Ni, Jie& Hu, Ping& Hu, Kai& Shi, Ying-Li. 2015. MiR-183 Regulates ITGB1P Expression and Promotes Invasion of Endometrial Stromal Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055103
Modern Language Association (MLA)
Chen, Jie…[et al.]. MiR-183 Regulates ITGB1P Expression and Promotes Invasion of Endometrial Stromal Cells. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1055103
American Medical Association (AMA)
Chen, Jie& Gu, Lin& Ni, Jie& Hu, Ping& Hu, Kai& Shi, Ying-Li. MiR-183 Regulates ITGB1P Expression and Promotes Invasion of Endometrial Stromal Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055103
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055103