Neem (Azadirachta indica A. Juss) Oil to Tackle Enteropathogenic Escherichia coli
Joint Authors
Del Serrone, Paola
Toniolo, Chiara
Nicoletti, Marcello
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-03
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Neem (Azadirachta indica A.
Juss) oil (NO) was assayed against forty-eight isolates of Escherichia coli by standardised disc diffusion test and microdilution test.
By molecular biology characterization, fourteen isolates resulted in diarrheagenic E.
coli with sixteen primer pairs that specifically amplify unique sequences of virulence genes and of 16S rRNA.
The NO showed biological activity against all isolates.
The bacterial growth inhibition zone by disc diffusion method (100 µL NO) ranged between 9.50 ± 0.70 and 30.00 ± 1.00 mm.
The antibacterial activity was furthermore determined at lower NO concentrations (1 : 10–1 : 10,000).
The percent of growth reduction ranged between 23.71 ± 1.00 and 99.70 ± 1.53.
The highest bacterial growth reduction was 1 : 10 NO concentration with 50 µL of bacterial suspension (ca.
1 × 106 CFU/mL).
There is significant difference between the antibacterial activities against pathogenic and nonpathogenic E.
coli, as well as NO and ciprofloxacin activities.
Viable cells after the different NO concentration treatments were checked by molecular biology assay using PMA dye.
On the basis of the obtained results, NO counteracts E.
coli and also influences the virulence of E.
coli viable cells after NO treatment.
The NO metabolomic composition was obtained using fingerprint HPTLC.
American Psychological Association (APA)
Del Serrone, Paola& Toniolo, Chiara& Nicoletti, Marcello. 2015. Neem (Azadirachta indica A. Juss) Oil to Tackle Enteropathogenic Escherichia coli. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055124
Modern Language Association (MLA)
Del Serrone, Paola…[et al.]. Neem (Azadirachta indica A. Juss) Oil to Tackle Enteropathogenic Escherichia coli. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1055124
American Medical Association (AMA)
Del Serrone, Paola& Toniolo, Chiara& Nicoletti, Marcello. Neem (Azadirachta indica A. Juss) Oil to Tackle Enteropathogenic Escherichia coli. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055124
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055124