The Effect of C-X-C Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling
Joint Authors
Li, Chunyan
Kang, Dong
Sun, Xiguang
Liu, Yufei
Wang, Jinhong
Gao, Pujun
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-16
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Objects.
To investigate the effect of CXCL13 (C-X-C motif chemokine 13) on hepatocellular carcinoma and clarify the potential mechanisms.
Methods.
32 patients with hepatocellular carcinoma and 12 healthy controls were recruited for analyzing the expression of CXCL13 by RT-PCR (reverse transcription-polymerase chain reaction).
ELISA (enzyme-linked immune-sorbent assay) was used to test the concentration of serum CXCL13.
The interaction between CXCL13 and Wnt signaling was analyzed by western blot.
In vitro PBMCs cultured with HepG2 supernatant, the levels of IL-12, IL4, IL-6, and IL-17, and four IgG subclasses were detected by ELISA.
Results.
The rate of high expression CXCL13 was 63.4% in advanced HCC patients, and the serum CXCL13 was also at a high level in stage IV HCC patients.
Meanwhile CXCL13 level was positively correlated with serum ALT (Alanine Transaminase) and AST (Aspartate Aminotransferase).
CXCL13 and Wnt/β-catenin signaling shared a positive feedback loop.
Furthermore, CXCL13 could obviously promote the expressions of IL-12 and IL-17, and induce IgG4 secreted by B cells.
Conclusions.
The effect of CXCL13 on promoting liver cancer is related to the activation of Wnt/β-catenin pathway and the facilitation of IL-12, IL-17 and IgG4.
CXCL13 plays an important role in the progression of HCC, and it may act as a potential target for the diagnosis and treatment of HCC.
American Psychological Association (APA)
Li, Chunyan& Kang, Dong& Sun, Xiguang& Liu, Yufei& Wang, Jinhong& Gao, Pujun. 2015. The Effect of C-X-C Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055130
Modern Language Association (MLA)
Li, Chunyan…[et al.]. The Effect of C-X-C Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1055130
American Medical Association (AMA)
Li, Chunyan& Kang, Dong& Sun, Xiguang& Liu, Yufei& Wang, Jinhong& Gao, Pujun. The Effect of C-X-C Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055130
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055130