Duck RIG-I CARD Domain Induces the Chicken IFN-β by Activating NF-κB
Joint Authors
Chen, Guohong
Chen, Yang
Huang, Zhengyang
Wang, Bin
Yu, Qinming
Liu, Ran
Xu, Qi
Ding, Jiatong
Chen, Guohong
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-30
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Retinoic acid-inducible gene I- (RIG-I-) like receptors (RLRs) have recently been identified as cytoplasmic sensors for viral RNA.
RIG-I, a member of RLRs family, plays an important role in innate immunity.
Although previous investigations have proved that RIG-I is absent in chickens, it remains largely unknown whether the chicken can respond to RIG-I ligand.
In this study, the eukaryotic expression vectors encoding duRIG-I full length (duck RIG-I, containing all domains), duRIG-I N-terminal (containing the two caspase activation and recruitment domain, CARDs), and duRIG-I C-terminal (containing helicase and regulatory domains) labeled with 6*His tags were constructed successfully and detected by western blotting.
Luciferase reporter assay and enzyme-linked immunosorbent assay (ELISA) detected the duRIG-I significantly activated NF-κB and induced the expression of IFN-β when polyinosinic-polycytidylic acid (poly[I:C], synthetic double-stranded RNA) challenges chicken embryonic fibroblasts cells (DF1 cells), while the duRIG-I was inactive in the absence of poly[I:C].
Further analysis revealed that the CARDs (duRIG-I-N) induced IFN-β production regardless of the presence of poly[I:C], while the CARD-lacking duRIG-I (duRIG-I-C) was not capable of activating downstream signals.
These results indicate that duRIG-I CARD domain plays an important role in the induction of IFN-β and provide a basis for further studying the function of RIG-I in avian innate immunity.
American Psychological Association (APA)
Chen, Yang& Huang, Zhengyang& Wang, Bin& Yu, Qinming& Liu, Ran& Xu, Qi…[et al.]. 2015. Duck RIG-I CARD Domain Induces the Chicken IFN-β by Activating NF-κB. BioMed Research International،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1055142
Modern Language Association (MLA)
Chen, Yang…[et al.]. Duck RIG-I CARD Domain Induces the Chicken IFN-β by Activating NF-κB. BioMed Research International No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1055142
American Medical Association (AMA)
Chen, Yang& Huang, Zhengyang& Wang, Bin& Yu, Qinming& Liu, Ran& Xu, Qi…[et al.]. Duck RIG-I CARD Domain Induces the Chicken IFN-β by Activating NF-κB. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1055142
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055142