In Vitro Bactericidal Activity of 4- and 5-Chloro-2-hydroxy-N-[1-oxo-1-(phenylamino)alkan-2-yl]benzamides against MRSA
Joint Authors
Vinsova, Jarmila
Zadrazilova, Iveta
Pospisilova, Sarka
Pauk, Karel
Cizek, Alois
Imramovsky, Aleš
Jampilek, Josef
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-15
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A series of nine substituted 2-hydroxy-N-[1-oxo-1-(phenylamino)alkan-2-yl]benzamides was assessed as prospective bactericidal agents against three clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) and S.
aureus ATCC 29213 as the reference and quality control strain.
The minimum bactericidal concentration was determined by subculturing aliquots from MIC determination onto substance-free agar plates.
The bactericidal kinetics of compounds 5-chloro-2-hydroxy-N-[(2S)-3-methyl-1-oxo-1-{[4-(trifluoromethyl)phenyl]amino}butan-2-yl]benzamide (1f), N-{(2S)-1-[(4-bromophenyl)amino]-3-methyl-1-oxobutan-2-yl}-4-chloro-2-hydroxybenzamide (1g), and 4-chloro-N-{(2S)-1-[(3,4-dichlorophenyl)amino]-3-methyl-1-oxobutan-2-yl}-2-hydroxybenzamide (1h) was established by time-kill assay with a final concentration of the compound equal to 1x, 2x, and 4x MIC; aliquots were removed at 0, 4, 6, 8, and 24 h time points.
The most potent bactericidal agent was compound 1f exhibiting remarkable rapid concentration-dependent bactericidal effect even at 2x MIC at 4, 6, and 8 h (with a reduction in bacterial count ranging from 3.08 to 3.75 log10 CFU/mL) and at 4x MIC at 4, 6, 8, and 24 h (5.30 log10 CFU/mL reduction in bacterial count) after incubation against MRSA 63718.
Reliable bactericidal effect against other strains was maintained at 4x MIC at 24 h.
American Psychological Association (APA)
Zadrazilova, Iveta& Pospisilova, Sarka& Pauk, Karel& Imramovsky, Aleš& Vinsova, Jarmila& Cizek, Alois…[et al.]. 2015. In Vitro Bactericidal Activity of 4- and 5-Chloro-2-hydroxy-N-[1-oxo-1-(phenylamino)alkan-2-yl]benzamides against MRSA. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055144
Modern Language Association (MLA)
Zadrazilova, Iveta…[et al.]. In Vitro Bactericidal Activity of 4- and 5-Chloro-2-hydroxy-N-[1-oxo-1-(phenylamino)alkan-2-yl]benzamides against MRSA. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1055144
American Medical Association (AMA)
Zadrazilova, Iveta& Pospisilova, Sarka& Pauk, Karel& Imramovsky, Aleš& Vinsova, Jarmila& Cizek, Alois…[et al.]. In Vitro Bactericidal Activity of 4- and 5-Chloro-2-hydroxy-N-[1-oxo-1-(phenylamino)alkan-2-yl]benzamides against MRSA. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055144
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055144