Macrophage-Targeting Gene Delivery Using a Micelle Composed of Mannose-Modified Lipid with Triazole Ring and Dioleoyl Trimethylammonium Propane
Joint Authors
Fukuda, Ichiki
Mochizuki, Shinichi
Sakurai, Kazuo
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-05
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Gene carriers with cell specific ligand molecules are needed for the treatment of several diseases.
Mannose is known to be recognized and incorporated into the cells through mannose recognition lectins that are exclusively expressed on macrophages.
In this study, we synthesized two types of mannose-modified lipids with different stereoisomer (α-mannose and β-mannose).
To make a complex with plasmid DNA (pDNA), termed “lipoplex,” we prepared a two-component micelle made from cationic lipid; dioleoyltrimethylammoniumpropane (DOTAP); and mannose-modified lipid (D/α-Man or D/β-Man).
The prepared D/α-Man lipoplexes were able to bind to one of the α-mannose lectins concanavalin A (ConA) immobilized on gold substrate in the quartz-crystal microbalance sensor cell.
D/β-Man lipoplexes did not show any frequency changes.
These results indicate that the mannose residues were exposed on the lipoplexes, leading to not only the binding to ConA but also the prevention of nonspecific interactions with proteins.
Both lipoplexes showed high transfection efficiencies to RAW264.7 cells which have several kinds of mannose lectins.
This delivery system to macrophages may overcome the problems for gene therapy and may be used for the treatment of immune diseases involved in macrophages.
American Psychological Association (APA)
Fukuda, Ichiki& Mochizuki, Shinichi& Sakurai, Kazuo. 2015. Macrophage-Targeting Gene Delivery Using a Micelle Composed of Mannose-Modified Lipid with Triazole Ring and Dioleoyl Trimethylammonium Propane. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055149
Modern Language Association (MLA)
Fukuda, Ichiki…[et al.]. Macrophage-Targeting Gene Delivery Using a Micelle Composed of Mannose-Modified Lipid with Triazole Ring and Dioleoyl Trimethylammonium Propane. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1055149
American Medical Association (AMA)
Fukuda, Ichiki& Mochizuki, Shinichi& Sakurai, Kazuo. Macrophage-Targeting Gene Delivery Using a Micelle Composed of Mannose-Modified Lipid with Triazole Ring and Dioleoyl Trimethylammonium Propane. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055149
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055149