Efficient and Specific Detection of Salmonella in Food Samples Using a stn-Based Loop-Mediated Isothermal Amplification Method
Joint Authors
Panbangred, Watanalai
Srisawat, Mevaree
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-12
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The Salmonella enterotoxin (stn) gene exhibits high homology among S.
enterica serovars and S.
bongori.
A set of 6 specific primers targeting the stn gene were designed for detection of Salmonella spp.
using the loop-mediated isothermal amplification (LAMP) method.
The primers amplified target sequences in all 102 strains of 87 serovars of Salmonella tested and no products were detected in 57 non-Salmonella strains.
The detection limit in pure cultures was 5 fg DNA/reaction when amplified at 65°C for 25 min.
The LAMP assay could detect Salmonella in artificially contaminated food samples as low as 220 cells/g of food without a preenrichment step.
However, the sensitivity was increased 100-fold (~2 cells/g) following 5 hr preenrichment at 35°C.
The LAMP technique, with a preenrichment step for 5 and 16 hr, was shown to give 100% specificity with food samples compared to the reference culture method in which 67 out of 90 food samples gave positive results.
Different food matrixes did not interfere with LAMP detection which employed a simple boiling method for DNA template preparation.
The results indicate that the LAMP method, targeting the stn gene, has great potential for detection of Salmonella in food samples with both high specificity and high sensitivity.
American Psychological Association (APA)
Srisawat, Mevaree& Panbangred, Watanalai. 2015. Efficient and Specific Detection of Salmonella in Food Samples Using a stn-Based Loop-Mediated Isothermal Amplification Method. BioMed Research International،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1055172
Modern Language Association (MLA)
Srisawat, Mevaree& Panbangred, Watanalai. Efficient and Specific Detection of Salmonella in Food Samples Using a stn-Based Loop-Mediated Isothermal Amplification Method. BioMed Research International No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1055172
American Medical Association (AMA)
Srisawat, Mevaree& Panbangred, Watanalai. Efficient and Specific Detection of Salmonella in Food Samples Using a stn-Based Loop-Mediated Isothermal Amplification Method. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1055172
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055172