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Identification of Reference Genes in Human Myelomonocytic Cells for Gene Expression Studies in Altered Gravity
Joint Authors
Thiel, Cora S.
Hauschild, Swantje
Tauber, Svantje
Paulsen, Katrin
Raig, Christiane
Raem, Arnold
Biskup, Josefine
Gutewort, Annett
Hürlimann, Eva
Unverdorben, Felix
Buttron, Isabell
Lauber, Beatrice
Philpot, Claudia
Lier, Hartwin
Engelmann, Frank
Layer, Liliana E.
Ullrich, Oliver
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-20, 20 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-13
Country of Publication
Egypt
No. of Pages
20
Main Subjects
Abstract EN
Gene expression studies are indispensable for investigation and elucidation of molecular mechanisms.
For the process of normalization, reference genes (“housekeeping genes”) are essential to verify gene expression analysis.
Thus, it is assumed that these reference genes demonstrate similar expression levels over all experimental conditions.
However, common recommendations about reference genes were established during 1 g conditions and therefore their applicability in studies with altered gravity has not been demonstrated yet.
The microarray technology is frequently used to generate expression profiles under defined conditions and to determine the relative difference in expression levels between two or more different states.
In our study, we searched for potential reference genes with stable expression during different gravitational conditions (microgravity, normogravity, and hypergravity) which are additionally not altered in different hardware systems.
We were able to identify eight genes (ALB, B4GALT6, GAPDH, HMBS, YWHAZ, ABCA5, ABCA9, and ABCC1) which demonstrated no altered gene expression levels in all tested conditions and therefore represent good candidates for the standardization of gene expression studies in altered gravity.
American Psychological Association (APA)
Thiel, Cora S.& Hauschild, Swantje& Tauber, Svantje& Paulsen, Katrin& Raig, Christiane& Raem, Arnold…[et al.]. 2015. Identification of Reference Genes in Human Myelomonocytic Cells for Gene Expression Studies in Altered Gravity. BioMed Research International،Vol. 2015, no. 2015, pp.1-20.
https://search.emarefa.net/detail/BIM-1055198
Modern Language Association (MLA)
Thiel, Cora S.…[et al.]. Identification of Reference Genes in Human Myelomonocytic Cells for Gene Expression Studies in Altered Gravity. BioMed Research International No. 2015 (2015), pp.1-20.
https://search.emarefa.net/detail/BIM-1055198
American Medical Association (AMA)
Thiel, Cora S.& Hauschild, Swantje& Tauber, Svantje& Paulsen, Katrin& Raig, Christiane& Raem, Arnold…[et al.]. Identification of Reference Genes in Human Myelomonocytic Cells for Gene Expression Studies in Altered Gravity. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-20.
https://search.emarefa.net/detail/BIM-1055198
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055198