Regulation of Osteoblast Differentiation by Acid-Etched andor Grit-Blasted Titanium Substrate Topography Is Enhanced by 1,25(OH)2D3 in a Sex-Dependent Manner
Joint Authors
Olivares-Navarrete, Rene
Hyzy, Sharon L.
Boyan, Barbara D.
Schwartz, Zvi
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-06
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
This study assessed contributions of micron-scale topography on clinically relevant titanium (Ti) to differentiation of osteoprogenitor cells and osteoblasts; the interaction of this effect with 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3); and if the effects are sex-dependent.
Male and female rat bone marrow cells (BMCs) were cultured on acid-etched (A, Ra=0.87 μm), grit-blasted (GB, Ra=3.90 μm), or grit-blasted/acid-etched (SLA, Ra=3.22 μm) Ti.
BMCs were sensitive to surface topography and underwent osteoblast differentiation.
This was greatest on SLA; acid etching and grit blasting contributed additively.
Primary osteoblasts were also sensitive to SLA, with less effect from individual structural components, demonstrated by enhanced local factor production.
Sex-dependent responses of BMCs to topography varied with parameter whereas male and female osteoblasts responded similarly to surface treatment.
1α,25(OH)2D3 enhanced cell responses on all surfaces similarly.
Effects were sex-dependent and male cells grown on a complex microstructured surface were much more sensitive than female cells.
These results indicate that effects of the complex SLA topography are greater than acid etching or grit blasting alone on multipotent BMCs and committed osteoblasts and that individual parameters are sex-specific.
The effect of 1α,25(OH)2D3 was sex dependent.
The results also suggest that levels of 1α,25(OH)2D3 in the patient may be important in osseointegration.
American Psychological Association (APA)
Olivares-Navarrete, Rene& Hyzy, Sharon L.& Boyan, Barbara D.& Schwartz, Zvi. 2015. Regulation of Osteoblast Differentiation by Acid-Etched andor Grit-Blasted Titanium Substrate Topography Is Enhanced by 1,25(OH)2D3 in a Sex-Dependent Manner. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1055203
Modern Language Association (MLA)
Olivares-Navarrete, Rene…[et al.]. Regulation of Osteoblast Differentiation by Acid-Etched andor Grit-Blasted Titanium Substrate Topography Is Enhanced by 1,25(OH)2D3 in a Sex-Dependent Manner. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1055203
American Medical Association (AMA)
Olivares-Navarrete, Rene& Hyzy, Sharon L.& Boyan, Barbara D.& Schwartz, Zvi. Regulation of Osteoblast Differentiation by Acid-Etched andor Grit-Blasted Titanium Substrate Topography Is Enhanced by 1,25(OH)2D3 in a Sex-Dependent Manner. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1055203
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055203