Comparison of Primary Models to Predict Microbial Growth by the Plate Count and Absorbance Methods
Joint Authors
Pla, María-Leonor
Oltra, Sandra
Esteban, María-Dolores
Andreu, Santiago
Palop, Alfredo
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-11
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
The selection of a primary model to describe microbial growth in predictive food microbiology often appears to be subjective.
The objective of this research was to check the performance of different mathematical models in predicting growth parameters, both by absorbance and plate count methods.
For this purpose, growth curves of three different microorganisms (Bacillus cereus, Listeria monocytogenes, and Escherichia coli) grown under the same conditions, but with different initial concentrations each, were analysed.
When measuring the microbial growth of each microorganism by optical density, almost all models provided quite high goodness of fit (r2>0.93) for all growth curves.
The growth rate remained approximately constant for all growth curves of each microorganism, when considering one growth model, but differences were found among models.
Three-phase linear model provided the lowest variation for growth rate values for all three microorganisms.
Baranyi model gave a variation marginally higher, despite a much better overall fitting.
When measuring the microbial growth by plate count, similar results were obtained.
These results provide insight into predictive microbiology and will help food microbiologists and researchers to choose the proper primary growth predictive model.
American Psychological Association (APA)
Pla, María-Leonor& Oltra, Sandra& Esteban, María-Dolores& Andreu, Santiago& Palop, Alfredo. 2015. Comparison of Primary Models to Predict Microbial Growth by the Plate Count and Absorbance Methods. BioMed Research International،Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1055204
Modern Language Association (MLA)
Pla, María-Leonor…[et al.]. Comparison of Primary Models to Predict Microbial Growth by the Plate Count and Absorbance Methods. BioMed Research International No. 2015 (2015), pp.1-14.
https://search.emarefa.net/detail/BIM-1055204
American Medical Association (AMA)
Pla, María-Leonor& Oltra, Sandra& Esteban, María-Dolores& Andreu, Santiago& Palop, Alfredo. Comparison of Primary Models to Predict Microbial Growth by the Plate Count and Absorbance Methods. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1055204
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055204