Label-Free Quantitative Mass Spectrometry Reveals a Panel of Differentially Expressed Proteins in Colorectal Cancer
Joint Authors
Gao, Chun-Fang
Gao, Jiang-Ling
Liu, Yan
Song, Wei
Zhang, Zhan-Yang
Fan, Nai-Jun
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-28
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
To identify potential biomarkers involved in CRC, a shotgun proteomic method was applied to identify soluble proteins in three CRCs and matched normal mucosal tissues using high-performance liquid chromatography and mass spectrometry.
Label-free protein profiling of three CRCs and matched normal mucosal tissues were then conducted to quantify and compare proteins.
Results showed that 67 of the 784 identified proteins were linked to CRC (28 upregulated and 39 downregulated).
Gene Ontology and DAVID databases were searched to identify the location and function of differential proteins that were related to the biological processes of binding, cell structure, signal transduction, cell adhesion, and so on.
Among the differentially expressed proteins, tropomyosin-3 (TPM3), endoplasmic reticulum resident protein 29 (ERp29), 18 kDa cationic antimicrobial protein (CAMP), and heat shock 70 kDa protein 8 (HSPA8) were verified to be upregulated in CRC tissue and seven cell lines through western blot analysis.
Furthermore, the upregulation of TPM3, ERp29, CAMP, and HSPA8 was validated in 69 CRCs byimmunohistochemistry (IHC) analysis.
Combination of TPM3, ERp29, CAMP, and HSPA8 can identify CRC from matched normal mucosal achieving an accuracy of 73.2% using IHC score.
These results suggest that TPM3, ERp29, CAMP, and HSPA8 are great potential IHC diagnostic biomarkers for CRC.
American Psychological Association (APA)
Fan, Nai-Jun& Gao, Jiang-Ling& Liu, Yan& Song, Wei& Zhang, Zhan-Yang& Gao, Chun-Fang. 2015. Label-Free Quantitative Mass Spectrometry Reveals a Panel of Differentially Expressed Proteins in Colorectal Cancer. BioMed Research International،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1055206
Modern Language Association (MLA)
Fan, Nai-Jun…[et al.]. Label-Free Quantitative Mass Spectrometry Reveals a Panel of Differentially Expressed Proteins in Colorectal Cancer. BioMed Research International No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1055206
American Medical Association (AMA)
Fan, Nai-Jun& Gao, Jiang-Ling& Liu, Yan& Song, Wei& Zhang, Zhan-Yang& Gao, Chun-Fang. Label-Free Quantitative Mass Spectrometry Reveals a Panel of Differentially Expressed Proteins in Colorectal Cancer. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1055206
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055206