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Binding and Endocytosis of Bovine Hololactoferrin by the Parasite Entamoeba histolytica
Joint Authors
Ortíz-Estrada, Guillermo
Calderón-Salinas, Víctor
León-Sicairos, Nidia
de la Garza, Mireya
Shibayama, Mineko
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-18
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Entamoeba histolytica is a human parasite that requires iron (Fe) for its metabolic function and virulence.
Bovine lactoferrin (B-Lf) and its peptides can be found in the digestive tract after dairy products are ingested.
The aim of this study was to compare virulent trophozoites recently isolated from hamster liver abscesses with nonvirulent trophozoites maintained for more than 30 years in cultures in vitro regarding their interaction with iron-charged B-Lf (B-holo-Lf).
We performed growth kinetics analyses of trophozoites in B-holo-Lf and throughout several consecutive transfers.
The virulent parasites showed higher growth and tolerance to iron than nonvirulent parasites.
Both amoeba variants specifically bound B-holo-Lf with a similar K d.
However, averages of 9.45 × 105 and 6.65 × 106 binding sites/cell were found for B-holo-Lf in nonvirulent and virulent amoebae, respectively.
Virulent amoebae bound more efficiently to human and bovine holo-Lf, human holo-transferrin, and human and bovine hemoglobin than nonvirulent amoebae.
Virulent amoebae showed two types of B-holo-Lf binding proteins.
Although both amoebae endocytosed this glycoprotein through clathrin-coated vesicles, the virulent amoebae also endocytosed B-holo-Lf through a cholesterol-dependent mechanism.
Both amoeba variants secreted cysteine proteases cleaving B-holo-Lf.
These data demonstrate that the B-Lf endocytosis is more efficient in virulent amoebae.
American Psychological Association (APA)
Ortíz-Estrada, Guillermo& Calderón-Salinas, Víctor& Shibayama, Mineko& León-Sicairos, Nidia& de la Garza, Mireya. 2015. Binding and Endocytosis of Bovine Hololactoferrin by the Parasite Entamoeba histolytica. BioMed Research International،Vol. 2015, no. 2015, pp.1-15.
https://search.emarefa.net/detail/BIM-1055240
Modern Language Association (MLA)
Ortíz-Estrada, Guillermo…[et al.]. Binding and Endocytosis of Bovine Hololactoferrin by the Parasite Entamoeba histolytica. BioMed Research International No. 2015 (2015), pp.1-15.
https://search.emarefa.net/detail/BIM-1055240
American Medical Association (AMA)
Ortíz-Estrada, Guillermo& Calderón-Salinas, Víctor& Shibayama, Mineko& León-Sicairos, Nidia& de la Garza, Mireya. Binding and Endocytosis of Bovine Hololactoferrin by the Parasite Entamoeba histolytica. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-15.
https://search.emarefa.net/detail/BIM-1055240
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055240