Hypoxia-Inducible Factor 1 Is an Inductor of Transcription Factor Activating Protein 2 Epsilon Expression during Chondrogenic Differentiation
Joint Authors
Kujat, Richard
Angele, Peter
Niebler, Stephan
Bosserhoff, Anja K.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-27
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
The transcription factor AP-2ε (activating enhancer-binding protein epsilon) is expressed in cartilage of humans and mice.
However, knowledge about regulatory mechanisms influencing AP-2ε expression is limited.
Using quantitative real time PCR, we detected a significant increase in AP-2ε mRNA expression comparing initial and late stages of chondrogenic differentiation processes in vitro and in vivo.
Interestingly, in these samples the expression pattern of the prominent hypoxia marker gene angiopoietin-like 4 (Angptl4) strongly correlated with that of AP-2ε suggesting that hypoxia might represent an external regulator of AP-2ε expression in mammals.
In order to show this, experiments directly targeting the activity of hypoxia-inducible factor-1 (HIF1), the complex mediating responses to oxygen deprivation, were performed.
While the HIF1-activating compounds 2,2′-dipyridyl and desferrioxamine resulted in significantly enhanced mRNA concentration of AP-2ε, siRNA against HIF1α led to a significantly reduced expression rate of AP-2ε .
Additionally, we detected a significant upregulation of the AP-2ε mRNA level after oxygen deprivation.
In sum, these different experimental approaches revealed a novel role for the HIF1 complex in the regulation of the AP-2ε gene in cartilaginous cells and underlined the important role of hypoxia as an important external regulatory stimulus during chondrogenic differentiation modulating the expression of downstream transcription factors.
American Psychological Association (APA)
Niebler, Stephan& Angele, Peter& Kujat, Richard& Bosserhoff, Anja K.. 2015. Hypoxia-Inducible Factor 1 Is an Inductor of Transcription Factor Activating Protein 2 Epsilon Expression during Chondrogenic Differentiation. BioMed Research International،Vol. 2015, no. 2015, pp.1-15.
https://search.emarefa.net/detail/BIM-1055254
Modern Language Association (MLA)
Niebler, Stephan…[et al.]. Hypoxia-Inducible Factor 1 Is an Inductor of Transcription Factor Activating Protein 2 Epsilon Expression during Chondrogenic Differentiation. BioMed Research International No. 2015 (2015), pp.1-15.
https://search.emarefa.net/detail/BIM-1055254
American Medical Association (AMA)
Niebler, Stephan& Angele, Peter& Kujat, Richard& Bosserhoff, Anja K.. Hypoxia-Inducible Factor 1 Is an Inductor of Transcription Factor Activating Protein 2 Epsilon Expression during Chondrogenic Differentiation. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-15.
https://search.emarefa.net/detail/BIM-1055254
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055254