Polymorphonuclear Leukocyte Apoptosis Is Accelerated by Sulfatides or Sulfatides-Treated Salmonella Typhimurium Bacteria
Joint Authors
Grishina, Zoryana V.
Viryasova, Galina M.
Romanova, Yulia M.
Sud’ina, Galina F.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Neutrophils die by apoptosis following activation and uptake of microbes or enter apoptosis spontaneously at the end of their lifespan if they do not encounter a pathogen.
Here we report that sulfatides or sulfatides-treated Salmonella Typhimurium bacteria accelerated human neutrophil apoptosis.
Neutrophil apoptosis was examined by flow cytometry.
Sulfatides caused prominent increase in percentage of apoptotic cells after 2.5 hrs of incubation.
Salmonella Typhimurium bacteria by themselves did not affect the basal level of apoptosis in neutrophil population.
When neutrophils were added to S.
Typhimurium “opsonized” by sulfatides, apoptotic index significantly increased, whereas the number of phagocyting cells was not influenced.
Sulfatides’ proapoptotic effect was strongly dependent on the activity of β-galactosidase; inhibition of this enzyme impaired its potency to accelerate apoptosis.
These data support the mechanism of neutrophil apoptosis triggering based on sulfatides’ ability to accumulate in intracellular compartments and mediate successive increase in ceramide content resulting from β-galactosidase activity.
American Psychological Association (APA)
Grishina, Zoryana V.& Viryasova, Galina M.& Romanova, Yulia M.& Sud’ina, Galina F.. 2015. Polymorphonuclear Leukocyte Apoptosis Is Accelerated by Sulfatides or Sulfatides-Treated Salmonella Typhimurium Bacteria. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055256
Modern Language Association (MLA)
Grishina, Zoryana V.…[et al.]. Polymorphonuclear Leukocyte Apoptosis Is Accelerated by Sulfatides or Sulfatides-Treated Salmonella Typhimurium Bacteria. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1055256
American Medical Association (AMA)
Grishina, Zoryana V.& Viryasova, Galina M.& Romanova, Yulia M.& Sud’ina, Galina F.. Polymorphonuclear Leukocyte Apoptosis Is Accelerated by Sulfatides or Sulfatides-Treated Salmonella Typhimurium Bacteria. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055256
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055256