Study of Cytotoxic Effects of Benzonitrile Pesticides
Joint Authors
Lovecká, Petra
Ruml, T.
Thimova, Marketa
Grznarova, Petra
Lipov, Jan
Knejzlik, Zdenek
Stiborova, Hana
Nindhia, Tjokorda Gde Tirta
Demnerova, Katerina
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-03
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The benzonitrile herbicides bromoxynil, chloroxynil, dichlobenil, and ioxynil have been used actively worldwide to control weeds in agriculture since 1970s.
Even though dichlobenil is prohibited in EU since 2008, studies addressing the fate of benzonitrile herbicides in the environment show that some metabolites of these herbicides are very persistent.
We tested the cytotoxic effects of benzonitrile herbicides and their microbial metabolites using two human cell lines, Hep G2 and HEK293T, representing liver and kidneys as potential target organs in humans.
The cell viability and proliferation were determined by MTT test and RTCA DP Analyzer system, respectively.
The latter allows real-time monitoring of the effect of added substances.
As the cytotoxic compounds could compromise cell membrane integrity, the lactate dehydrogenase test was performed as well.
We observed high toxic effects of bromoxynil, chloroxynil, and ioxynil on both tested cell lines.
In contrast, we determined only low inhibition of cell growth in presence of dichlobenil and microbial metabolites originating from the tested herbicides.
American Psychological Association (APA)
Lovecká, Petra& Thimova, Marketa& Grznarova, Petra& Lipov, Jan& Knejzlik, Zdenek& Stiborova, Hana…[et al.]. 2015. Study of Cytotoxic Effects of Benzonitrile Pesticides. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1055257
Modern Language Association (MLA)
Lovecká, Petra…[et al.]. Study of Cytotoxic Effects of Benzonitrile Pesticides. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1055257
American Medical Association (AMA)
Lovecká, Petra& Thimova, Marketa& Grznarova, Petra& Lipov, Jan& Knejzlik, Zdenek& Stiborova, Hana…[et al.]. Study of Cytotoxic Effects of Benzonitrile Pesticides. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1055257
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055257