Characterization of Red Blood Cells with Multiwavelength Transmission Spectroscopy
Joint Authors
Serebrennikova, Yulia M.
Huffman, Debra E.
Garcia-Rubio, Luis H.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-12
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Multiwavelength transmission (MWT) spectroscopy was applied to the investigation of the morphological parameters and composition of red blood cells (RBCs).
The MWT spectra were quantitatively analyzed with a Mie theory based interpretation model modified to incorporate the effects of the nonsphericity and orientation of RBCs.
The MWT spectra of the healthy and anemic samples were investigated for the RBC indices in open and blinded studies.
When MWT performance was evaluated against a standard reference system, very good agreement between two methods, with R2>0.85 for all indices studied, was demonstrated.
The RBC morphological parameters were used to characterize three types of anemia and to draw an association between RBC morphology and anemia severity.
The MWT spectra of RBCs infected with malaria parasite Plasmodium falciparum at different life cycle stages were analyzed for RBC morphological parameters.
The changes in the RBC volume, surface area, aspect ratio, and hemoglobin composition were used to trace the morphological and compositional alterations in the infected RBCs occurring with parasites’ development and to provide insights into parasite-host interactions.
The MWT method was shown to be reliable for determination of the RBC morphological parameters and to be valuable for identification of the RBC pathologic changes and disease states.
American Psychological Association (APA)
Serebrennikova, Yulia M.& Huffman, Debra E.& Garcia-Rubio, Luis H.. 2015. Characterization of Red Blood Cells with Multiwavelength Transmission Spectroscopy. BioMed Research International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1055266
Modern Language Association (MLA)
Serebrennikova, Yulia M.…[et al.]. Characterization of Red Blood Cells with Multiwavelength Transmission Spectroscopy. BioMed Research International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1055266
American Medical Association (AMA)
Serebrennikova, Yulia M.& Huffman, Debra E.& Garcia-Rubio, Luis H.. Characterization of Red Blood Cells with Multiwavelength Transmission Spectroscopy. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1055266
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055266