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Senescence-Associated Changes in Proteome and O-GlcNAcylation Pattern in Human Peritoneal Mesothelial Cells
Joint Authors
Aufricht, Christoph
Witowski, Janusz
Rudolf, Andras
Herzog, Rebecca
Tarantino, Silvia
Kratochwill, Klaus
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-10
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Introduction.
Senescence of peritoneal mesothelial cells represents a biological program defined by arrested cell growth and altered cell secretory phenotype with potential impact in peritoneal dialysis.
This study aims to characterize cellular senescence at the level of global protein expression profiles and modification of proteins with O-linked N-acetylglucosamine (O-GlcNAcylation).
Methods.
A comparative proteomics analysis between young and senescent human peritoneal mesothelial cells (HPMC) was performed using two-dimensional gel electrophoresis.
O-GlcNAc status was assessed by Western blot under normal conditions and after modulation with 6-diazo-5-oxo-L-norleucine (DON) to decrease O-GlcNAcylation or O-(2-acetamido-2-deoxy-D-glucopyranosylidene) amino N-phenyl carbamate (PUGNAc) to increase O-GlcNAcylation.
Results.
Comparison of protein pattern of senescent and young HPMC revealed 29 differentially abundant protein spots, 11 of which were identified to be actin (cytoplasmic 1 and 2), cytokeratin-7, cofilin-2, transgelin-2, Hsp60, Hsc70, proteasome β-subunits (type-2 and type-3), nucleoside diphosphate kinase A, and cytosolic 5′(3′)-deoxyribonucleotidase.
Although the global level of O-GlcNAcylation was comparable, senescent cells were not sensitive to modulation by PUGNAc.
Discussion.
This study identified changes of the proteome and altered dynamics of O-GlcNAc regulation in senescent mesothelial cells.
Whereas changes in cytoskeleton-associated proteins likely reflect altered cell morphology, changes in chaperoning and housekeeping proteins may have functional impact on cellular stress response in peritoneal dialysis.
American Psychological Association (APA)
Herzog, Rebecca& Tarantino, Silvia& Rudolf, Andras& Aufricht, Christoph& Kratochwill, Klaus& Witowski, Janusz. 2015. Senescence-Associated Changes in Proteome and O-GlcNAcylation Pattern in Human Peritoneal Mesothelial Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1055267
Modern Language Association (MLA)
Herzog, Rebecca…[et al.]. Senescence-Associated Changes in Proteome and O-GlcNAcylation Pattern in Human Peritoneal Mesothelial Cells. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1055267
American Medical Association (AMA)
Herzog, Rebecca& Tarantino, Silvia& Rudolf, Andras& Aufricht, Christoph& Kratochwill, Klaus& Witowski, Janusz. Senescence-Associated Changes in Proteome and O-GlcNAcylation Pattern in Human Peritoneal Mesothelial Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1055267
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055267