DNaseI Protects against Paraquat-Induced Acute Lung Injury and Pulmonary Fibrosis Mediated by Mitochondrial DNA
Joint Authors
Li, Guo
Yuzhen, Li
Xiaoxiang, Chen
Wei, Zhou
Changqing, Zhu
Shuang, Ye
Chen, Yi
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-11
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
Paraquat (PQ) poisoning is a lethal toxicological challenge that served as a disease model of acute lung injury and pulmonary fibrosis, but the mechanism is undetermined and no effective treatment has been discovered.
Methods and Findings.
We demonstrated that PQ injures mitochondria and leads to mtDNA release.
The mtDNA mediated PBMC recruitment and stimulated the alveolar epithelial cell production of TGF-β1 in vitro.
The levels of mtDNA in circulation and bronchial alveolar lavage fluid (BALF) were elevated in a mouse of PQ-induced lung injury.
DNaseI could protect PQ-induced lung injury and significantly improved survival.
Acute lung injury markers, such as TNFα, IL-1β, and IL-6, and marker of fibrosis, collagen I, were downregulated in parallel with the elimination of mtDNA by DNaseI.
These data indicate a possible mechanism for PQ-induced, mtDNA-mediated lung injury, which may be shared by other causes of lung injury, as suggested by the same protective effect of DNaseI in bleomycin-induced lung injury model.
Interestingly, increased mtDNA in the BALF of patients with amyopathic dermatomyositis-interstitial lung disease can be appreciated.
Conclusions.
DNaseI targeting mtDNA may be a promising approach for the treatment of PQ-induced acute lung injury and pulmonary fibrosis that merits fast tracking through clinical trials.
American Psychological Association (APA)
Li, Guo& Yuzhen, Li& Chen, Yi& Xiaoxiang, Chen& Wei, Zhou& Changqing, Zhu…[et al.]. 2015. DNaseI Protects against Paraquat-Induced Acute Lung Injury and Pulmonary Fibrosis Mediated by Mitochondrial DNA. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055280
Modern Language Association (MLA)
Li, Guo…[et al.]. DNaseI Protects against Paraquat-Induced Acute Lung Injury and Pulmonary Fibrosis Mediated by Mitochondrial DNA. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1055280
American Medical Association (AMA)
Li, Guo& Yuzhen, Li& Chen, Yi& Xiaoxiang, Chen& Wei, Zhou& Changqing, Zhu…[et al.]. DNaseI Protects against Paraquat-Induced Acute Lung Injury and Pulmonary Fibrosis Mediated by Mitochondrial DNA. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055280
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055280