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Current and New Approaches in GMO Detection: Challenges and Solutions
Joint Authors
Fraiture, Marie-Alice
Herman, Philippe
Taverniers, Isabel
De Loose, Marc
Deforce, Dieter
Roosens, Nancy H. C.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-22, 22 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-15
Country of Publication
Egypt
No. of Pages
22
Main Subjects
Abstract EN
In many countries, genetically modified organisms (GMO) legislations have been established in order to guarantee the traceability of food/feed products on the market and to protect the consumer freedom of choice.
Therefore, several GMO detection strategies, mainly based on DNA, have been developed to implement these legislations.
Due to its numerous advantages, the quantitative PCR (qPCR) is the method of choice for the enforcement laboratories in GMO routine analysis.
However, given the increasing number and diversity of GMO developed and put on the market around the world, some technical hurdles could be encountered with the qPCR technology, mainly owing to its inherent properties.
To address these challenges, alternative GMO detection methods have been developed, allowing faster detections of single GM target (e.g., loop-mediated isothermal amplification), simultaneous detections of multiple GM targets (e.g., PCR capillary gel electrophoresis, microarray, and Luminex), more accurate quantification of GM targets (e.g., digital PCR), or characterization of partially known (e.g., DNA walking and Next Generation Sequencing (NGS)) or unknown (e.g., NGS) GMO.
The benefits and drawbacks of these methods are discussed in this review.
American Psychological Association (APA)
Fraiture, Marie-Alice& Herman, Philippe& Taverniers, Isabel& De Loose, Marc& Deforce, Dieter& Roosens, Nancy H. C.. 2015. Current and New Approaches in GMO Detection: Challenges and Solutions. BioMed Research International،Vol. 2015, no. 2015, pp.1-22.
https://search.emarefa.net/detail/BIM-1055301
Modern Language Association (MLA)
Fraiture, Marie-Alice…[et al.]. Current and New Approaches in GMO Detection: Challenges and Solutions. BioMed Research International No. 2015 (2015), pp.1-22.
https://search.emarefa.net/detail/BIM-1055301
American Medical Association (AMA)
Fraiture, Marie-Alice& Herman, Philippe& Taverniers, Isabel& De Loose, Marc& Deforce, Dieter& Roosens, Nancy H. C.. Current and New Approaches in GMO Detection: Challenges and Solutions. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-22.
https://search.emarefa.net/detail/BIM-1055301
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055301