Quantitative iTRAQ LC-MSMS Proteomics Reveals the Proteome Profiles of DF-1 Cells after Infection with Subgroup J Avian Leukosis Virus
Joint Authors
Li, Xiaofei
Wang, Qi
Gao, Yanni
Qi, Xiaole
Wang, Yongqiang
Gao, Honglei
Gao, Yulong
Wang, Xiaomei
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-08
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Avian leukosis virus subgroup J (ALV-J) is an avian oncogenic retrovirus that can induce various clinical tumors and has caused severe economic losses in China.
To improve our understanding of the host cellular responses to virus infection and the pathogenesis of ALV-J infection, we applied isobaric tags for relative and absolute quantification (iTRAQ) labeling coupled with multidimensional liquid chromatography-tandem mass spectrometry to detect the protein changes in DF-1 cells infected and mock-infected with ALV-J.
A total of 75 cellular proteins were significantly changed, including 33 upregulated proteins and 42 downregulated proteins.
The reliability of iTRAQ-LC MS/MS was confirmed via real-time PCR.
Most of these proteins were related to the physiological functions of metabolic processes, biosynthetic processes, responses to stimuli, protein binding, signal transduction, cell cytoskeleton, and so forth.
We also found some proteins that play important roles in apoptosis and oncogenicity.
The differentially expressed proteins identified may provide valuable information to elucidate the pathogenesis of virus infection and virus-host interactions.
American Psychological Association (APA)
Li, Xiaofei& Wang, Qi& Gao, Yanni& Qi, Xiaole& Wang, Yongqiang& Gao, Honglei…[et al.]. 2015. Quantitative iTRAQ LC-MSMS Proteomics Reveals the Proteome Profiles of DF-1 Cells after Infection with Subgroup J Avian Leukosis Virus. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055316
Modern Language Association (MLA)
Li, Xiaofei…[et al.]. Quantitative iTRAQ LC-MSMS Proteomics Reveals the Proteome Profiles of DF-1 Cells after Infection with Subgroup J Avian Leukosis Virus. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1055316
American Medical Association (AMA)
Li, Xiaofei& Wang, Qi& Gao, Yanni& Qi, Xiaole& Wang, Yongqiang& Gao, Honglei…[et al.]. Quantitative iTRAQ LC-MSMS Proteomics Reveals the Proteome Profiles of DF-1 Cells after Infection with Subgroup J Avian Leukosis Virus. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055316
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055316