Unravelling the RNA-Binding Properties of SAFB Proteins in Breast Cancer Cells
Joint Authors
Tyson-Capper, Alison
Hong, Elaine
Best, Andrew
Chin, Jas
Razdan, Anshuli
Curk, Tomaz
Elliott, David J.
Gautrey, Hannah
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-26
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Scaffold attachment factor B1 (SAFB1) and SAFB2 proteins are oestrogen (ER) corepressors that bind to and modulate ER activity through chromatin remodelling or interaction with the basal transcription machinery.
SAFB proteins also have an internal RNA-recognition motif but little is known about the RNA-binding properties of SAFB1 or SAFB2.
We utilised crosslinking and immunoprecipitation (iCLIP) coupled with high-throughput sequencing to enable a transcriptome-wide mapping of SAFB1 protein-RNA interactions in breast cancer MCF-7 cells.
Analysis of crosslinking frequency mapped to transcript regions revealed that SAFB1 binds to coding and noncoding RNAs (ncRNAs).
The highest proportion of SAFB1 crosslink sites mapped to ncRNAs, followed by intergenic regions, open reading frames (ORFs), introns, and 3′ or 5′ untranslated regions (UTR).
Furthermore, we reveal that SAFB1 binds directly to RNA and its binding is particularly enriched at purine-rich sequences not dissimilar to the RNA-binding motifs for SR proteins.
Using RNAi, we also show, for the first time, that single depletion of either SAFB1 or SAFB2 leads to an increase in expression of the other SAFB protein in both MCF-7 and MDA-MD231 breast cancer cells.
American Psychological Association (APA)
Hong, Elaine& Best, Andrew& Gautrey, Hannah& Chin, Jas& Razdan, Anshuli& Curk, Tomaz…[et al.]. 2015. Unravelling the RNA-Binding Properties of SAFB Proteins in Breast Cancer Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1055318
Modern Language Association (MLA)
Hong, Elaine…[et al.]. Unravelling the RNA-Binding Properties of SAFB Proteins in Breast Cancer Cells. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1055318
American Medical Association (AMA)
Hong, Elaine& Best, Andrew& Gautrey, Hannah& Chin, Jas& Razdan, Anshuli& Curk, Tomaz…[et al.]. Unravelling the RNA-Binding Properties of SAFB Proteins in Breast Cancer Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1055318
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055318