التقدير الطيفي للثيامين بالاقتران التأكسدي مع بارا امينو فينول بوجود بيريودات البوتاسيوم

Abstract EN

A spectral method was developed for the estimation of easy and highly sensitive thiamine based on the direct estimation of thiamine through the reaction of oxidative coupling with the para-amino phenol reagent at a concentration of 1 x10-2 molar with the oxidation factor of potassium periods at a concentration of 3 x 10-2 molar in the center of the function (11.11), with a yellow orange color dissolved in water and stabilized, giving the highest absorption at 441 nm wavelength.

The BIR method is followed in the range of 60-2.4 μg.

ML-1, with a correlation coefficient of 0.9986, and the molar absorbance 8.526 ×103 liter.

Mol-1.

cm-1, and Sandal marker 0.0390 μg.

cm-2, the relative standard deviation does not exceed 3.21%, and the detection limit is 1.001 μg.

ML- 1.

The method was successful in estimating thiamine in pharmaceuticals, and ranged between 99-99.9%.