The Absence of N-Acetyl-D-glucosamine Causes Attenuation of Virulence of Candida albicans upon Interaction with Vaginal Epithelial Cells In Vitro
Joint Authors
Ördögh, Lilla
Vörös, Andrea
Nagy, István
Kondorosi, Éva
Manczinger, Máté
Bocsik, Alexandra
Kocsis, Gabriella F.
Hegedűs, Zoltán
Marton, Annamária
Vízler, Csaba
Tubak, Vilmos
Deli, Mária
Lakatos, Lóránt
Kemény, Lajos
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-20
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
To better understand the molecular events underlying vulvovaginal candidiasis, we established an in vitro system.
Immortalized vaginal epithelial cells were infected with live, yeast form C.
albicans and C.
albicans cultured in the same medium without vaginal epithelial cells were used as control.
In both cases a yeast to hyphae transition was robustly induced.
Whole transcriptome sequencing was used to identify specific gene expression changes in C.
albicans.
Numerous genes leading to a yeast to hyphae transition and hyphae specific genes were upregulated in the control hyphae and the hyphae in response to vaginal epithelial cells.
Strikingly, the GlcNAc pathway was exclusively triggered by vaginal epithelial cells.
Functional analysis in our in vitro system revealed that the GlcNAc biosynthesis is involved in the adherence to, and the ability to kill, vaginal epithelial cells in vitro, thus indicating the key role for this pathway in the virulence of C.
albicans upon vulvovaginal candidiasis.
American Psychological Association (APA)
Manczinger, Máté& Bocsik, Alexandra& Kocsis, Gabriella F.& Vörös, Andrea& Hegedűs, Zoltán& Ördögh, Lilla…[et al.]. 2015. The Absence of N-Acetyl-D-glucosamine Causes Attenuation of Virulence of Candida albicans upon Interaction with Vaginal Epithelial Cells In Vitro. BioMed Research International،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1055331
Modern Language Association (MLA)
Manczinger, Máté…[et al.]. The Absence of N-Acetyl-D-glucosamine Causes Attenuation of Virulence of Candida albicans upon Interaction with Vaginal Epithelial Cells In Vitro. BioMed Research International No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1055331
American Medical Association (AMA)
Manczinger, Máté& Bocsik, Alexandra& Kocsis, Gabriella F.& Vörös, Andrea& Hegedűs, Zoltán& Ördögh, Lilla…[et al.]. The Absence of N-Acetyl-D-glucosamine Causes Attenuation of Virulence of Candida albicans upon Interaction with Vaginal Epithelial Cells In Vitro. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1055331
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055331