Immunodiagnosis of Canine Visceral Leishmaniasis Using Mimotope Peptides Selected from Phage Displayed Combinatorial Libraries
Joint Authors
Fujiwara, Ricardo T.
Toledo-Machado, Christina Monerat
Machado de Avila, Ricardo Andrez
NGuyen, Christophe
Granier, Claude
Bueno, Lilian Lacerda
Carneiro, Claudia Martins
Menezes-Souza, Daniel
Carneiro, Rubens Antonio
Chávez-Olórtegui, Carlos
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-29
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
ELISA and RIFI are currently used for serodiagnosis of canine visceral leishmaniasis (CVL).
The accuracy of these tests is controversial in endemic areas where canine infections by Trypanosoma cruzi may occur.
We evaluated the usefulness of synthetic peptides that were selected through phage display technique in the serodiagnosis of CVL.
Peptides were chosen based on their ability to bind to IgGs purified from infected dogs pooled sera.
We selected three phage clones that reacted only with those IgGs.
Peptides were synthesized, polymerized with glutaraldehyde, and used as antigens in ELISA assays.
Each individual peptide or a mix of them was reactive with infected dogs serum.
The assay was highly sensitive and specific when compared to soluble Leishmania antigen that showed cross-reactivity with anti-T.
cruzi IgGs.
Our results demonstrate that phage display technique is useful for selection of peptides that may represent valuable synthetic antigens for an improved serodiagnosis of CVL.
American Psychological Association (APA)
Toledo-Machado, Christina Monerat& Machado de Avila, Ricardo Andrez& NGuyen, Christophe& Granier, Claude& Bueno, Lilian Lacerda& Carneiro, Claudia Martins…[et al.]. 2015. Immunodiagnosis of Canine Visceral Leishmaniasis Using Mimotope Peptides Selected from Phage Displayed Combinatorial Libraries. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055335
Modern Language Association (MLA)
Toledo-Machado, Christina Monerat…[et al.]. Immunodiagnosis of Canine Visceral Leishmaniasis Using Mimotope Peptides Selected from Phage Displayed Combinatorial Libraries. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1055335
American Medical Association (AMA)
Toledo-Machado, Christina Monerat& Machado de Avila, Ricardo Andrez& NGuyen, Christophe& Granier, Claude& Bueno, Lilian Lacerda& Carneiro, Claudia Martins…[et al.]. Immunodiagnosis of Canine Visceral Leishmaniasis Using Mimotope Peptides Selected from Phage Displayed Combinatorial Libraries. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055335
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055335