Puerarin Inhibits oxLDL-Induced Macrophage Activation and Foam Cell Formation in Human THP1 Macrophage
Joint Authors
Zhang, Heng
Zhai, Zhenhua
Zhou, Hongyu
Li, Yao
Li, Xiaojie
Lin, Yuhan
Li, Weihong
Shi, Yueping
Zhou, Ming-Sheng
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-21
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Puerarin, an isoflavone derived from Kudzu roots, has been widely used for treatment of cardiovascular and cerebral vascular diseases in China and other Asian countries.
However, the underlying mechanisms are largely unknown.
The present study investigated whether puerarin inhibited atherogenic lipid oxLDL-mediated macrophage activation and foam cell formation in human THP1 macrophage.
Treatment with oxLDL significantly increased the mRNA expression of proinflammatory cytokines tumor necrosis factor α (TNFα, 160%) and interleukin (IL) 1β (13 fold) accompanied by upregulation of toll-like receptor 4 (TLR4, 165%) and the ratio of phospho-IκBα/IκBα in THP1 macrophage.
Puerarin dose-dependently prevented an increase in oxLDL-induced proinflammatory gene expression with downregulation of TLR4 and the ratio of phospho-IκBα/IκBα.
Furthermore, puerarin prevented oxLDL-mediated lipid deposition and foam cell formation associated with downregulation of scavenger receptor CD36.
Flow cytometry analysis showed that puerarin reduced the number of early apoptotic cells of macrophages induced by oxLDL.
Our results show that puerarin has anti-inflammatory and antiatherogenic effects in vitro; the underlying mechanisms may involve the inhibition of TLR4/NFκB pathway and downregulation of CD36 expression.
The results from the present study provide scientific evidence and may expand our armamentarium to use puerarin for prevention and treatment of cardiovascular and atherosclerotic diseases.
American Psychological Association (APA)
Zhang, Heng& Zhai, Zhenhua& Zhou, Hongyu& Li, Yao& Li, Xiaojie& Lin, Yuhan…[et al.]. 2015. Puerarin Inhibits oxLDL-Induced Macrophage Activation and Foam Cell Formation in Human THP1 Macrophage. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055350
Modern Language Association (MLA)
Zhang, Heng…[et al.]. Puerarin Inhibits oxLDL-Induced Macrophage Activation and Foam Cell Formation in Human THP1 Macrophage. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1055350
American Medical Association (AMA)
Zhang, Heng& Zhai, Zhenhua& Zhou, Hongyu& Li, Yao& Li, Xiaojie& Lin, Yuhan…[et al.]. Puerarin Inhibits oxLDL-Induced Macrophage Activation and Foam Cell Formation in Human THP1 Macrophage. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055350
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055350