A Multiplex PCR for Simultaneous Detection of Three Zoonotic Parasites Ancylostoma ceylanicum, A. caninum, and Giardia lamblia Assemblage A
Joint Authors
Tan, Liping
Li, Guoqing
Wu, Sheng
Yu, Xingang
Abullahi, Auwalu Yusuf
Song, Meiran
Wang, Zhen
Jiang, Biao
Hu, Wei
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-31
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Ancylostoma ceylanicum, A.
caninum, and Giardia lamblia assemblage A are common intestinal parasites of dogs and cats; they can also infect humans, causing parasitic zoonoses.
In this study, a multiplex PCR method was developed for simultaneous identification and detection of those three zoonotic parasites.
Three pairs of specific primers were designed based on ITS sequence of A.
ceylanicum and A.
caninum and TPI gene of G.
lamblia available in the GenBank.
The multiplex PCR reaction system was established by optimizing the reaction condition, and a series of tests on the sensitivity, specificity, and clinical application were also conducted.
Results showed that three target fragments were amplified specifically; the detection limit was 10 eggs for both A.
ceylanicum and A.
caninum, 72 pg DNA for G.
lamblia.
Of 112 clinical fecal samples, 34.8% and 17.8% samples were positive for A.
caninum and A.
ceylanicum, respectively, while only 2.7% samples were positive for G.
lamblia assemblage A.
It is concluded that the established multiplex PCR assay is a convenient, rapid, cost-effective, and high-efficiency method for molecular detection and epidemiological investigation of three zoonotic parasites.
American Psychological Association (APA)
Hu, Wei& Wu, Sheng& Yu, Xingang& Abullahi, Auwalu Yusuf& Song, Meiran& Tan, Liping…[et al.]. 2015. A Multiplex PCR for Simultaneous Detection of Three Zoonotic Parasites Ancylostoma ceylanicum, A. caninum, and Giardia lamblia Assemblage A. BioMed Research International،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1055360
Modern Language Association (MLA)
Hu, Wei…[et al.]. A Multiplex PCR for Simultaneous Detection of Three Zoonotic Parasites Ancylostoma ceylanicum, A. caninum, and Giardia lamblia Assemblage A. BioMed Research International No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1055360
American Medical Association (AMA)
Hu, Wei& Wu, Sheng& Yu, Xingang& Abullahi, Auwalu Yusuf& Song, Meiran& Tan, Liping…[et al.]. A Multiplex PCR for Simultaneous Detection of Three Zoonotic Parasites Ancylostoma ceylanicum, A. caninum, and Giardia lamblia Assemblage A. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1055360
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055360