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Low-Molecular-Weight Polyethyleneimine Grafted Polythiophene for Efficient siRNA Delivery
Joint Authors
Ito, Yoshihiro
He, Pan
Hagiwara, Kyoji
Chong, Hui
Yu, Hsiao-hua
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-11
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Owing to its hydrophilicity, negative charge, small size, and labile degradation by endogenous nucleases, small interfering RNA (siRNA) delivery must be achieved by a carrier system.
In this study, cationic copolymers composed of low-molecular-weight polyethylenimine and polythiophenes were synthesized and evaluated as novel self-tracking siRNA delivery vectors.
The concept underlying the design of these copolymers is that hydrophobicity and rigidity of polythiophenes should enhance the transport of siRNA across the cell membrane and endosomal membrane.
A gel retardation assay showed that the nanosized complexes formed between the copolymers and siRNA were stable even at a molar ratio of 1 : 2.
The high cellular uptake (>80%) and localization of the copolymer vectors inside the cells were easily analyzed by tracking the fluorescence of polythiophene using fluorescent microscopy and cytometry.
An in vitro luciferase knockdown (KD) assay in A549-luc cells demonstrated that the siRNA complexes with more hydrophobic copolymers achieved a higher KD efficiency of 52.8% without notable cytotoxicity, indicating protein-specific KD activity rather than solely the cytotoxicity of the materials.
Our polythiophene copolymers should serve as novel, efficient, low cell toxicity, and label-free siRNA delivery systems.
American Psychological Association (APA)
He, Pan& Hagiwara, Kyoji& Chong, Hui& Yu, Hsiao-hua& Ito, Yoshihiro. 2015. Low-Molecular-Weight Polyethyleneimine Grafted Polythiophene for Efficient siRNA Delivery. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1055363
Modern Language Association (MLA)
He, Pan…[et al.]. Low-Molecular-Weight Polyethyleneimine Grafted Polythiophene for Efficient siRNA Delivery. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1055363
American Medical Association (AMA)
He, Pan& Hagiwara, Kyoji& Chong, Hui& Yu, Hsiao-hua& Ito, Yoshihiro. Low-Molecular-Weight Polyethyleneimine Grafted Polythiophene for Efficient siRNA Delivery. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1055363
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055363