DNASynth: A Computer Program for Assembly of Artificial Gene Parts in Decreasing Temperature
Joint Authors
Wojtowicz-Krawiec, Anna
Plucienniczak, Andrzej
Nowak, Robert M.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-06
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Artificial gene synthesis requires consideration of nucleotide sequence development as well as long DNA molecule assembly protocols.
The nucleotide sequence of the molecule must meet many conditions including particular preferences of the host organism for certain codons, avoidance of specific regulatory subsequences, and a lack of secondary structures that inhibit expression.
The chemical synthesis of DNA molecule has limitations in terms of strand length; thus, the creation of artificial genes requires the assembly of long DNA molecules from shorter fragments.
In the approach presented, the algorithm and the computer program address both tasks: developing the optimal nucleotide sequence to encode a given peptide for a given host organism and determining the long DNA assembly protocol.
These tasks are closely connected; a change in codon usage may lead to changes in the optimal assembly protocol, and the lack of a simple assembly protocol may be addressed by changing the nucleotide sequence.
The computer program presented in this study was tested with real data from an experiment in a wet biological laboratory to synthesize a peptide.
The benefit of the presented algorithm and its application is the shorter time, compared to polymerase cycling assembly, needed to produce a ready synthetic gene.
American Psychological Association (APA)
Nowak, Robert M.& Wojtowicz-Krawiec, Anna& Plucienniczak, Andrzej. 2015. DNASynth: A Computer Program for Assembly of Artificial Gene Parts in Decreasing Temperature. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055387
Modern Language Association (MLA)
Nowak, Robert M.…[et al.]. DNASynth: A Computer Program for Assembly of Artificial Gene Parts in Decreasing Temperature. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1055387
American Medical Association (AMA)
Nowak, Robert M.& Wojtowicz-Krawiec, Anna& Plucienniczak, Andrzej. DNASynth: A Computer Program for Assembly of Artificial Gene Parts in Decreasing Temperature. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055387
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055387