Transgenic Carrot Expressing Fusion Protein Comprising M. tuberculosis Antigens Induces Immune Response in Mice
Joint Authors
Uvarova, Elena A.
Belavin, Pavel A.
Permyakova, Natalya V.
Zagorskaya, Alla A.
Deineko, Elena V.
Nosareva, Olesya V.
Nesterov, Andrey E.
Novikovskaya, Anna A.
Zav’yalov, Evgeniy L.
Moshkin, Mikhail P.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-09
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Tuberculosis remains one of the major infectious diseases, which continues to pose a major global health problem.
Transgenic plants may serve as bioreactors to produce heterologous proteins including antibodies, antigens, and hormones.
In the present study, a genetic construct has been designed that comprises the Mycobacterium tuberculosis genes cfp10, esat6 and dIFN gene, which encode deltaferon, a recombinant analog of the human γ-interferon designed for expression in plant tissues.
This construct was transferred to the carrot (Daucus carota L.) genome by Agrobacterium-mediated transformation.
This study demonstrates that the fusion protein CFP10-ESAT6-dIFN is synthesized in the transgenic carrot storage roots.
The protein is able to induce both humoral and cell-mediated immune responses in laboratory animals (mice) when administered either orally or by injection.
It should be emphasized that M.
tuberculosis antigens contained in the fusion protein have no cytotoxic effect on peripheral blood mononuclear cells.
American Psychological Association (APA)
Permyakova, Natalya V.& Zagorskaya, Alla A.& Belavin, Pavel A.& Uvarova, Elena A.& Nosareva, Olesya V.& Nesterov, Andrey E.…[et al.]. 2015. Transgenic Carrot Expressing Fusion Protein Comprising M. tuberculosis Antigens Induces Immune Response in Mice. BioMed Research International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1055408
Modern Language Association (MLA)
Permyakova, Natalya V.…[et al.]. Transgenic Carrot Expressing Fusion Protein Comprising M. tuberculosis Antigens Induces Immune Response in Mice. BioMed Research International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1055408
American Medical Association (AMA)
Permyakova, Natalya V.& Zagorskaya, Alla A.& Belavin, Pavel A.& Uvarova, Elena A.& Nosareva, Olesya V.& Nesterov, Andrey E.…[et al.]. Transgenic Carrot Expressing Fusion Protein Comprising M. tuberculosis Antigens Induces Immune Response in Mice. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1055408
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055408