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Sliding Motility, Biofilm Formation, and Glycopeptidolipid Production in Mycobacterium colombiense Strains
Joint Authors
Maya-Hoyos, Milena
Leguizamón, John
Mariño-Ramírez, Leonardo
Soto Ospina, Carlos Yesid
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-28
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Mycobacterium colombiense is a novel member of the Mycobacterium avium complex, which produces respiratory and disseminated infections in immunosuppressed patients.
Currently, the morphological and genetic bases underlying the phenotypic features of M.
colombiense strains remain unknown.
In the present study, we demonstrated that M.
colombiense strains displaying smooth morphology show increased biofilm formation on hydrophobic surfaces and sliding on motility plates.
Thin-layer chromatography experiments showed that M.
colombiense strains displaying smooth colonies produce large amounts of glycolipids with a chromatographic behaviour similar to that of the glycopeptidolipids (GPLs) of M.
avium.
Conversely, we observed a natural rough variant of M.
colombiense (57B strain) lacking pigmentation and exhibiting impaired sliding, biofilm formation, and GPL production.
Bioinformatics analyses revealed a gene cluster that is likely involved in GPL biosynthesis in M.
colombiense CECT 3035.
RT-qPCR experiments showed that motile culture conditions activate the transcription of genes possibly involved in key enzymatic activities of GPL biosynthesis.
American Psychological Association (APA)
Maya-Hoyos, Milena& Leguizamón, John& Mariño-Ramírez, Leonardo& Soto Ospina, Carlos Yesid. 2015. Sliding Motility, Biofilm Formation, and Glycopeptidolipid Production in Mycobacterium colombiense Strains. BioMed Research International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1055423
Modern Language Association (MLA)
Maya-Hoyos, Milena…[et al.]. Sliding Motility, Biofilm Formation, and Glycopeptidolipid Production in Mycobacterium colombiense Strains. BioMed Research International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1055423
American Medical Association (AMA)
Maya-Hoyos, Milena& Leguizamón, John& Mariño-Ramírez, Leonardo& Soto Ospina, Carlos Yesid. Sliding Motility, Biofilm Formation, and Glycopeptidolipid Production in Mycobacterium colombiense Strains. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1055423
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055423