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Organotypic Cultures of Intervertebral Disc Cells: Responses to Growth Factors and Signaling Pathways Involved
Joint Authors
Pratsinis, Harris
Kletsas, Dimitris
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-25
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Intervertebral disc (IVD) degeneration is strongly associated with low back pain, a major cause of disability worldwide.
An in-depth understanding of IVD cell physiology is required for the design of novel regenerative therapies.
Accordingly, aim of this work was the study of IVD cell responses to mitogenic growth factors in a three-dimensional (3D) organotypic milieu, comprising characteristic molecules of IVD’s extracellular matrix.
In particular, annulus fibrosus (AF) cells were cultured inside collagen type-I gels, while nucleus pulposus (NP) cells in chondroitin sulfate A (CSA) supplemented collagen gels, and the effects of Platelet-Derived Growth Factor (PDGF), basic Fibroblast Growth Factor (bFGF), and Insulin-Like Growth Factor-I (IGF-I) were assessed.
All three growth factors stimulated DNA synthesis in both AF and NP 3D cell cultures, with potencies similar to those observed previously in monolayers.
CSA supplementation inhibited basal DNA synthesis rates, without affecting the response to growth factors.
ERK and Akt were found to be phosphorylated following growth factor stimulation.
Blockade of these two signaling pathways using pharmacologic inhibitors significantly, though not completely, inhibited growth factor-induced DNA synthesis.
The proposed culture systems may prove useful for further in vitro studies aiming at future interventions for IVD regeneration.
American Psychological Association (APA)
Pratsinis, Harris& Kletsas, Dimitris. 2015. Organotypic Cultures of Intervertebral Disc Cells: Responses to Growth Factors and Signaling Pathways Involved. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055449
Modern Language Association (MLA)
Pratsinis, Harris& Kletsas, Dimitris. Organotypic Cultures of Intervertebral Disc Cells: Responses to Growth Factors and Signaling Pathways Involved. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1055449
American Medical Association (AMA)
Pratsinis, Harris& Kletsas, Dimitris. Organotypic Cultures of Intervertebral Disc Cells: Responses to Growth Factors and Signaling Pathways Involved. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055449
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055449