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The High PMNs Phagocytosis Resistance of Enterococcal Isolates from RTx Patients
Joint Authors
Witkowski, Jacek M.
Rutkowski, Bolesław
Daca, Agnieszka
Jarzembowski, Tomasz
Bryl, Ewa
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-10
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Infections caused by opportunistic pathogens such as enterococci remain difficult to manage, especially in immunocompromised patients.
Because of infections’ limited symptoms in such patients the additional problems are to find proper diagnostic criteria and the management of infection.
Here we aimed to compare the resistance of commensal enterococcal strains and RTx patients’ isolates, to PMNs phagocytosis.
Thirty-six enterococcal urine and faecal isolates from RTx patients and 17 faecal isolates from healthy volunteers were cultured in planktonic and biofilm forms in 37°C or 42°C.
Another tested variable was the addition of immunosuppressant to the culture media.
Bacterial cells were stained with fluorescent reporter (CFDA, PI) and incubated with PMNs.
Results of phagocytosis were estimated as a mean fluorescence intensity (MFI) of PMNs using flow cytometry.
Commensal enterococci cultured in all abovementioned (37°C and 42°C/the addition of immunosuppressant) conditions were less resistant to phagocytosis compared to RTx isolates.
Observed significant difference in phagocytosis resistance suggests that patients in immunosuppression are colonized with high risk strains which may lead to the development of infection.
American Psychological Association (APA)
Jarzembowski, Tomasz& Daca, Agnieszka& Witkowski, Jacek M.& Bryl, Ewa& Rutkowski, Bolesław. 2015. The High PMNs Phagocytosis Resistance of Enterococcal Isolates from RTx Patients. BioMed Research International،Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1055471
Modern Language Association (MLA)
Jarzembowski, Tomasz…[et al.]. The High PMNs Phagocytosis Resistance of Enterococcal Isolates from RTx Patients. BioMed Research International No. 2015 (2015), pp.1-5.
https://search.emarefa.net/detail/BIM-1055471
American Medical Association (AMA)
Jarzembowski, Tomasz& Daca, Agnieszka& Witkowski, Jacek M.& Bryl, Ewa& Rutkowski, Bolesław. The High PMNs Phagocytosis Resistance of Enterococcal Isolates from RTx Patients. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1055471
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055471