Potentiated Osteoinductivity via Cotransfection with BMP-2 and VEGF Genes in Microencapsulated C2C12 Cells
Joint Authors
Shen, Yang
Qiao, Han
Fan, Qiming
Zhang, Shuhong
Tang, Tingting
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-16
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Microcapsules with entrapped cells hold great promise for repairing bone defects.
Unfortunately, the osteoinductivity of microcapsules has been restricted by many factors, among which the deficiency of functional proteins is a significant priority.
We potentiated the osteoinductivity of microencapsulated cells via cotransfection with BMP-2 and VEGF genes.
Various tissue-derived mesenchymal stem cells and cell lines were compared for BMP-2 and VEGF cotransfection.
Ethidium bromide (EB)/Calcein AM staining revealed that all of the cell categories could survive for 4 weeks after microencapsulation.
An ELISA assay indicated that all microencapsulated BMP-2 or VEGF transfected cells could secrete gene products constitutively for 1 month.
Particularly, the recombinant microencapsulated C2C12 cells released the most desirable level of BMP-2 and VEGF.
Further experiments demonstrated that microencapsulated BMP-2 and VEGF cotransfected C2C12 cells generated both BMP-2 and VEGF for 4 weeks.
Additionally, the cotransfection of BMP-2 and VEGF in microencapsulated C2C12 cells showed a stronger osteogenic induction against BMSCs than individual BMP-2-transfected microencapsulated C2C12 cells.
These results demonstrated that the cotransfection of BMP-2 and VEGF into microencapsulated C2C12 cells is of potent utility for the potentiation of bone regeneration, which would provide a promising clinical strategy for cellular therapy in bone defects.
American Psychological Association (APA)
Shen, Yang& Qiao, Han& Fan, Qiming& Zhang, Shuhong& Tang, Tingting. 2015. Potentiated Osteoinductivity via Cotransfection with BMP-2 and VEGF Genes in Microencapsulated C2C12 Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055480
Modern Language Association (MLA)
Shen, Yang…[et al.]. Potentiated Osteoinductivity via Cotransfection with BMP-2 and VEGF Genes in Microencapsulated C2C12 Cells. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1055480
American Medical Association (AMA)
Shen, Yang& Qiao, Han& Fan, Qiming& Zhang, Shuhong& Tang, Tingting. Potentiated Osteoinductivity via Cotransfection with BMP-2 and VEGF Genes in Microencapsulated C2C12 Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055480
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055480