Analysis of Hereditary Elliptocytosis with Decreased Binding of Eosin-5-maleimide to Red Blood Cells
Joint Authors
Suemori, Shin-ichiro
Wada, Hideho
Nakanishi, Hidekazu
Tsujioka, Takayuki
Sugihara, Takashi
Tohyama, Kaoru
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-18
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Flow cytometric test for analyzing the eosin-5-maleimide (EMA) binding to red blood cells has been believed to be a specific method for diagnosing hereditary spherocytosis (HS).
However, it has been reported that diseases other than HS, such as hereditary pyropoikilocytosis (HPP) and Southeast Asian ovalocytosis (SAO), which are forms in the category of hereditary elliptocytosis (HE), show decreased EMA binding to red blood cells.
We analyzed EMA binding to red blood cells in 101 healthy control subjects and 42 HS patients and obtained a mean channel fluorescence (MCF) cut-off value of 36.4 (sensitivity 0.97, specificity 0.95).
Using this method, we also analyzed 12 HE patients.
Among them, four HE patients showed the MCF at or below the cut-off value.
It indicates that some HE patients have decreased EMA binding to red blood cells.
Two of these four HE patients were classified as common HE, and two were spherocytic HE with reduced spectrin.
This study demonstrates that, in addition to patients with HPP or SAO, some HE patients have decreased EMA binding to red blood cells.
American Psychological Association (APA)
Suemori, Shin-ichiro& Wada, Hideho& Nakanishi, Hidekazu& Tsujioka, Takayuki& Sugihara, Takashi& Tohyama, Kaoru. 2015. Analysis of Hereditary Elliptocytosis with Decreased Binding of Eosin-5-maleimide to Red Blood Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1055510
Modern Language Association (MLA)
Suemori, Shin-ichiro…[et al.]. Analysis of Hereditary Elliptocytosis with Decreased Binding of Eosin-5-maleimide to Red Blood Cells. BioMed Research International No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1055510
American Medical Association (AMA)
Suemori, Shin-ichiro& Wada, Hideho& Nakanishi, Hidekazu& Tsujioka, Takayuki& Sugihara, Takashi& Tohyama, Kaoru. Analysis of Hereditary Elliptocytosis with Decreased Binding of Eosin-5-maleimide to Red Blood Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1055510
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055510