Establishment of the MethyLight Assay for Assessing Aging, Cigarette Smoking, and Alcohol Consumption
Joint Authors
Endo, Kosuke
Li, Jiawei
Nakanishi, Michio
Asada, Takashi
Ikesue, Masahiro
Goto, Yoichi
Fukushima, Yasue
Iwai, Naoharu
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-22
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The environmental factors such as aging, smoking, and alcohol consumption have been reported to influence DNA methylation (DNAm).
However, the versatility of DNAm measurement by DNAm array systems is low in clinical use.
Thus, we developed the MethyLight assay as a simple method to measure DNAm.
In the present study, we isolated peripheral blood DNA from 33 healthy volunteers and selected cg25809905, cg02228185, and cg17861230 as aging, cg23576855 as smoking, and cg02583484 as alcohol consumption biomarkers.
The predicted age by methylation rates of cg25809905 and cg17861230 significantly correlated with chronological age.
In immortalized B-cells, DNAm rates of two sites showed a younger status than the chronological age of donor.
On the other hand, the predicted age of the patients with myocardial infarction (MI) was not accelerated.
The methylation rate of cg23576855 was able to discriminate the groups based on the smoking status.
The DNAm rate of cg02583484 was reduced in subjects with habitual alcohol consumption compared to that of subjects without habitual alcohol consumption.
In conclusion, our MethyLight assay system reconfirms that aging, smoking, and alcohol consumption influenced DNAm in peripheral blood in the Japanese.
This MethyLight system will facilitate DNAm measurement in epidemiological and clinical studies.
American Psychological Association (APA)
Endo, Kosuke& Li, Jiawei& Nakanishi, Michio& Asada, Takashi& Ikesue, Masahiro& Goto, Yoichi…[et al.]. 2015. Establishment of the MethyLight Assay for Assessing Aging, Cigarette Smoking, and Alcohol Consumption. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055512
Modern Language Association (MLA)
Endo, Kosuke…[et al.]. Establishment of the MethyLight Assay for Assessing Aging, Cigarette Smoking, and Alcohol Consumption. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1055512
American Medical Association (AMA)
Endo, Kosuke& Li, Jiawei& Nakanishi, Michio& Asada, Takashi& Ikesue, Masahiro& Goto, Yoichi…[et al.]. Establishment of the MethyLight Assay for Assessing Aging, Cigarette Smoking, and Alcohol Consumption. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055512
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055512