![](/images/graphics-bg.png)
Characterization of a Thioredoxin-1 Gene from Taenia solium and Its Encoding Product
Joint Authors
Ochoa-Sánchez, Alicia
Landa-Piedra, Abraham
Rodríguez-Lima, Oscar
Jiménez, Lucía
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-19
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Taenia solium thioredoxin-1 gene (TsTrx-1) has a length of 771 bp with three exons and two introns.
The core promoter gene presents two putative stress transcription factor binding sites, one putative TATA box, and a transcription start site (TSS).
TsTrx-1 mRNA is expressed higher in larvae than in adult.
This gene encodes a protein of 107 amino acids that presents the Trx active site (CGPC), the classical secondary structure of the thioredoxin fold, and the highest degree of identity with the Echinococcus granulosus Trx.
A recombinant TsTrx-1 (rTsTrx-1) was produced in Escherichia coli with redox activity.
Optimal activity for rTsTrx-1 was at pH 6.5 in the range of 15 to 25°C.
The enzyme conserved activity for 3 h and lost it in 24 h at 37°C.
rTsTrx-1 lost 50% activity after 1 h and lost activity completely in 24 h at temperatures higher than 55°C.
Best storage temperature for rTsTrx-1 was at −70°C.
It was inhibited by high concentrations of H2O2 and methylglyoxal (MG), but it was inhibited neither by NaCl nor by anti-rTsTrx-1 rabbit antibodies that strongly recognized a ~12 kDa band in extracts from several parasites.
These TsTrx-1 properties open the opportunity to study its role in relationship T.
solium-hosts.
American Psychological Association (APA)
Jiménez, Lucía& Rodríguez-Lima, Oscar& Ochoa-Sánchez, Alicia& Landa-Piedra, Abraham. 2015. Characterization of a Thioredoxin-1 Gene from Taenia solium and Its Encoding Product. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055517
Modern Language Association (MLA)
Jiménez, Lucía…[et al.]. Characterization of a Thioredoxin-1 Gene from Taenia solium and Its Encoding Product. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1055517
American Medical Association (AMA)
Jiménez, Lucía& Rodríguez-Lima, Oscar& Ochoa-Sánchez, Alicia& Landa-Piedra, Abraham. Characterization of a Thioredoxin-1 Gene from Taenia solium and Its Encoding Product. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055517
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055517