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The Comparative Utility of Viromer RED and Lipofectamine for Transient Gene Introduction into Glial Cells
Joint Authors
Rao, Sudheendra
Morales, Alejo A.
Pearse, Damien D.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-11
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The introduction of genes into glial cells for mechanistic studies of cell function and as a therapeutic for gene delivery is an expanding field.
Though viral vector based systems do exhibit good delivery efficiency and long-term production of the transgene, the need for transient gene expression, broad and rapid gene setup methodologies, and safety concerns regarding in vivo application still incentivize research into the use of nonviral gene delivery methods.
In the current study, aviral gene delivery vectors based upon cationic lipid (Lipofectamine 3000) lipoplex or polyethylenimine (Viromer RED) polyplex technologies were examined in cell lines and primary glial cells for their transfection efficiencies, gene expression levels, and toxicity.
The transfection efficiencies of polyplex and lipoplex agents were found to be comparable in a limited, yet similar, transfection setting, with or without serum across a number of cell types.
However, differential effects on cell-specific transgene expression and reduced viability with cargo loaded polyplex were observed.
Overall, our data suggests that polyplex technology could perform comparably to the market dominant lipoplex technology in transfecting various cells lines including glial cells but also stress a need for further refinement of polyplex reagents to minimize their effects on cell viability.
American Psychological Association (APA)
Rao, Sudheendra& Morales, Alejo A.& Pearse, Damien D.. 2015. The Comparative Utility of Viromer RED and Lipofectamine for Transient Gene Introduction into Glial Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055549
Modern Language Association (MLA)
Rao, Sudheendra…[et al.]. The Comparative Utility of Viromer RED and Lipofectamine for Transient Gene Introduction into Glial Cells. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1055549
American Medical Association (AMA)
Rao, Sudheendra& Morales, Alejo A.& Pearse, Damien D.. The Comparative Utility of Viromer RED and Lipofectamine for Transient Gene Introduction into Glial Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055549
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055549