The Effect of Primary Cancer Cell Culture Models on the Results of Drug Chemosensitivity Assays: The Application of Perfusion Microbioreactor System as Cell Culture Vessel
Joint Authors
Hsieh, Chia-Hsun
Chen, Yi-Dao
Huang, Shiang-Fu
Wang, Hung-Ming
Wu, Min-Hsien
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
To precisely and faithfully perform cell-based drug chemosensitivity assays, a well-defined and biologically relevant culture condition is required.
For the former, a perfusion microbioreactor system capable of providing a stable culture condition was adopted.
For the latter, however, little is known about the impact of culture models on the physiology and chemosensitivity assay results of primary oral cavity cancer cells.
To address the issues, experiments were performed.
Results showed that minor environmental pH change could significantly affect the metabolic activity of cells, demonstrating the importance of stable culture condition for such assays.
Moreover, the culture models could also significantly influence the metabolic activity and proliferation of cells.
Furthermore, the choice of culture models might lead to different outcomes of chemosensitivity assays.
Compared with the similar test based on tumor-level assays, the spheroid model could overestimate the drug resistance of cells to cisplatin, whereas the 2D and 3D culture models might overestimate the chemosensitivity of cells to such anticancer drug.
In this study, the 3D culture models with same cell density as that in tumor samples showed comparable chemosensitivity assay results as the tumor-level assays.
Overall, this study has provided some fundamental information for establishing a precise and faithful drug chemosensitivity assay.
American Psychological Association (APA)
Hsieh, Chia-Hsun& Chen, Yi-Dao& Huang, Shiang-Fu& Wang, Hung-Ming& Wu, Min-Hsien. 2015. The Effect of Primary Cancer Cell Culture Models on the Results of Drug Chemosensitivity Assays: The Application of Perfusion Microbioreactor System as Cell Culture Vessel. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055599
Modern Language Association (MLA)
Hsieh, Chia-Hsun…[et al.]. The Effect of Primary Cancer Cell Culture Models on the Results of Drug Chemosensitivity Assays: The Application of Perfusion Microbioreactor System as Cell Culture Vessel. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1055599
American Medical Association (AMA)
Hsieh, Chia-Hsun& Chen, Yi-Dao& Huang, Shiang-Fu& Wang, Hung-Ming& Wu, Min-Hsien. The Effect of Primary Cancer Cell Culture Models on the Results of Drug Chemosensitivity Assays: The Application of Perfusion Microbioreactor System as Cell Culture Vessel. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055599
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055599