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A Tissue Retrieval and Postharvest Processing Regimen for Rodent Reproductive Tissues Compatible with Long-Term Storage on the International Space Station and Postflight Biospecimen Sharing Program
Joint Authors
Gupta, Vijayalaxmi
Holets-Bondar, Lesya
Roby, Katherine F.
Enders, George
Tash, Joseph S.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-13
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Collection and processing of tissues to preserve space flight effects from animals after return to Earth is challenging.
Specimens must be harvested with minimal time after landing to minimize postflight readaptation alterations in protein expression/translation, posttranslational modifications, and expression, as well as changes in gene expression and tissue histological degradation after euthanasia.
We report the development of a widely applicable strategy for determining the window of optimal species-specific and tissue-specific posteuthanasia harvest that can be utilized to integrate into multi-investigator Biospecimen Sharing Programs.
We also determined methods for ISS-compatible long-term tissue storage (10 months at −80°C) that yield recovery of high quality mRNA and protein for western analysis after sample return.
Our focus was reproductive tissues.
The time following euthanasia where tissues could be collected and histological integrity was maintained varied with tissue and species ranging between 1 and 3 hours.
RNA quality was preserved in key reproductive tissues fixed in RNAlater up to 40 min after euthanasia.
Postfixation processing was also standardized for safe shipment back to our laboratory.
Our strategy can be adapted for other tissues under NASA’s Biospecimen Sharing Program or similar multi-investigator tissue sharing opportunities.
American Psychological Association (APA)
Gupta, Vijayalaxmi& Holets-Bondar, Lesya& Roby, Katherine F.& Enders, George& Tash, Joseph S.. 2015. A Tissue Retrieval and Postharvest Processing Regimen for Rodent Reproductive Tissues Compatible with Long-Term Storage on the International Space Station and Postflight Biospecimen Sharing Program. BioMed Research International،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1055620
Modern Language Association (MLA)
Gupta, Vijayalaxmi…[et al.]. A Tissue Retrieval and Postharvest Processing Regimen for Rodent Reproductive Tissues Compatible with Long-Term Storage on the International Space Station and Postflight Biospecimen Sharing Program. BioMed Research International No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1055620
American Medical Association (AMA)
Gupta, Vijayalaxmi& Holets-Bondar, Lesya& Roby, Katherine F.& Enders, George& Tash, Joseph S.. A Tissue Retrieval and Postharvest Processing Regimen for Rodent Reproductive Tissues Compatible with Long-Term Storage on the International Space Station and Postflight Biospecimen Sharing Program. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1055620
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055620