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In Vitro Propagation, Phytochemical Analysis, and Evaluation of Free Radical Scavenging Property of Scrophularia kakudensis Franch Tissue Extracts
Joint Authors
Jeong, Byoung Ryong
Manivannan, Abinaya
Soundararajan, Prabhakaran
Park, Yoo Gyeong
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-16
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The current study deals with in vitro propagation, antioxidant property estimation, and assessment of acacetin content in Scrophularia kakudensis Franch.
Adventitious shoot induction was achieved from the nodal explant with the highest number of adventitious shoots per explant (17.4) on Murashige and Skoog’s (MS) medium fortified with 2.0 mg·L−1 6-benzyladenine (BA) and 0.5 mg L−1 indole-3-acetic acid (IAA).
Maximum number of roots per plant (16.5) was noted in half strength MS medium supplemented with 0.5 mg·L−1 IAA.
The regenerated plants displayed successful survival ratio (95%) in the greenhouse.
The highest content of acacetin, a pharmaceutically important flavonoid, was observed in the shoot extracts (in vitro: 32.83 µg·g−1 FW; in vivo: 30.05 µg·g−1 FW) followed by root extracts.
Total phenol and flavonoid contents along with free radical scavenging assays revealed the occurrence of larger amount of antioxidants in shoot extract in comparison with callus and root extracts of S.
kakudensis.
Thus, the outcome of the present study can be highly beneficial for the germplasm conservation and commercial cultivation of S.
kakudensis for therapeutic purposes.
American Psychological Association (APA)
Manivannan, Abinaya& Soundararajan, Prabhakaran& Park, Yoo Gyeong& Jeong, Byoung Ryong. 2015. In Vitro Propagation, Phytochemical Analysis, and Evaluation of Free Radical Scavenging Property of Scrophularia kakudensis Franch Tissue Extracts. BioMed Research International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1055634
Modern Language Association (MLA)
Manivannan, Abinaya…[et al.]. In Vitro Propagation, Phytochemical Analysis, and Evaluation of Free Radical Scavenging Property of Scrophularia kakudensis Franch Tissue Extracts. BioMed Research International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1055634
American Medical Association (AMA)
Manivannan, Abinaya& Soundararajan, Prabhakaran& Park, Yoo Gyeong& Jeong, Byoung Ryong. In Vitro Propagation, Phytochemical Analysis, and Evaluation of Free Radical Scavenging Property of Scrophularia kakudensis Franch Tissue Extracts. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1055634
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055634