Lentivirus-Mediated siRNA Targeting ER-α Inhibits Tumorigenesis and Induces Apoptosis in Hepatocarcinoma Cells
Joint Authors
Cao, Jun
Jiang, Ping
Bai, Wen-Hui
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-27
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background and Objectives.
Estrogen receptor-α (ER-α) plays important roles in hepatocarcinogenesis.
Recent studies have shown that ER-α could lead to cell cycle progression or inhibition of apoptosis.
To better understand the role of ER-α, RNA interference (RNAi) was used to inhibit ER-α expression in the human hepatocellular carcinoma (HCC) cells.
Methods.
Lentivirus-mediated ER-α small interfering RNA (siRNA) was transfected into HCC cells Hep3B.
ER-α expression was monitored by real-time polymerase chain reaction (PCR) and western blot.
Cell proliferation, apoptosis, and invasion were examined by methyl thiazol tetrazolium (MTT), flow cytometry (FCM), and invasion assay, respectively.
Results.
ER-α siRNA efficiently downregulated the expression of ER-α in Hep3B cells at both mRNA and protein levels in a time-dependent manner.
ER-α siRNA also inhibited cell proliferation and reduced cell invasion (compared with other groups, P < 0.05 , resp.).
Furthermore, knockdown of ER-α slowed down the cell population at S phase and increased the rate of apoptosis ( P < 0.05 , resp.).
Conclusion.
ER-α knockdown suppressed the growth of HCC cells.
Thus, ER-α may play a very important role in carcinogenesis of HCC and its knockdown may offer a new potential gene therapy approach for human liver cancer in the future.
American Psychological Association (APA)
Jiang, Ping& Cao, Jun& Bai, Wen-Hui. 2015. Lentivirus-Mediated siRNA Targeting ER-α Inhibits Tumorigenesis and Induces Apoptosis in Hepatocarcinoma Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055680
Modern Language Association (MLA)
Jiang, Ping…[et al.]. Lentivirus-Mediated siRNA Targeting ER-α Inhibits Tumorigenesis and Induces Apoptosis in Hepatocarcinoma Cells. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1055680
American Medical Association (AMA)
Jiang, Ping& Cao, Jun& Bai, Wen-Hui. Lentivirus-Mediated siRNA Targeting ER-α Inhibits Tumorigenesis and Induces Apoptosis in Hepatocarcinoma Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055680
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055680