Effect of Air-Polishing on Titanium Surfaces, Biofilm Removal, and Biocompatibility: A Pilot Study

Abstract EN

Purpose.

The aims of this in vitro study were to evaluate morphological changes induced by glycine powder air-polishing on titanium surfaces, biofilm removal, and biocompatibility.

Material and Methods.

Titanium grade IV discs were allocated into two groups: (1) discs without biofilm and (2) discs for Streptococcus mutans biofilm formation.

Discs in each group were further subdivided into (a) no treatment and (b) air-polishing treatment with glycine powder.

Discs were characterized by scanning electron microscopy (SEM), electron-dispersive spectroscopy (EDS), and confocal microscopy.

Bacterial biofilms were quantified using a crystal violet dye-binding assay.

Biocompatibility was evaluated by measuring the coverage and viability of L929 fibroblast cells cultured on the discs.

Results.

Air-polishing increased the roughness of treated discs (P<0.05).

EDS analysis did not show significant differences in the chemical composition of treated and nontreated discs.

The amount of residual biofilm on treated discs was 8.6-fold lower than untreated controls (P<0.05).

Coverage of treated discs by fibroblasts was half that of untreated discs (P<0.05) although both groups had the same cell viability.

Conclusions.

Air-polishing removed a significant amount of biofilm from titanium surfaces.

The “polishing” was accompanied by increased surface roughness, but there were no changes in chemical and elemental compositions, nor the biocompatibility.