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A Novel Anti-Histone H1 Monoclonal Antibody, SSV Monoclonal Antibody, Improves Lung Injury and Survival in a Mouse Model of Lipopolysaccharide-Induced Sepsis-Like Syndrome
Joint Authors
Nakano, Toshiaki
Kusano, Toru
Chiang, Kuei-Chen
Inomata, Masafumi
Shimada, Yayoi
Ohmori, Naoya
Goto, Takeshi
Sato, Shuji
Kawamoto, Seiji
Takaoka, Yuki
Shiraishi, Norio
Noguchi, Takayuki
Kitano, Seigo
Goto, Shigeru
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-11
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
Histones play important roles in both host defenses and inflammation related to microbial infection.
A peptide mimotope (SSV) was identified from a novel histone H1 monoclonal antibody (16G9 mAb) that was shown to inhibit the mixed lymphocyte reaction.
In the present study, an anti-SSV producing hybridoma was established.
We investigated the effects of SSV mAb in a mouse acute inflammation model induced by intraperitoneal injection of lipopolysaccharide (LPS).
Methods.
SSV mAb was generated and characterized.
Mice were treated with SSV mAb or a control IgG antibody prior to LPS injection.
Evaluation of survival rate and lung tissue on histological score was performed.
The levels of inflammatory cytokines and histones H1, H3, and H4 in plasma and lung tissue were measured by ELISA.
Results.
Competitive ELISA revealed that SSV mAb binds to histone H1.
SSV mAb improved lung injury and prolonged the survival of LPS-injected mice.
Increased levels of histones H1, H3, and H4 and inflammatory cytokines (TNF-α, IL-1β, and IL-6) in plasma and lung tissue after LPS injection were ameliorated by SSV mAb.
Conclusion.
SSV mAb is shown to have anti-inflammatory activity and organ-protective effects, highlighting the importance of controlling histone H1 as well as H3 and H4 levels during inflammation.
American Psychological Association (APA)
Kusano, Toru& Chiang, Kuei-Chen& Inomata, Masafumi& Shimada, Yayoi& Ohmori, Naoya& Goto, Takeshi…[et al.]. 2015. A Novel Anti-Histone H1 Monoclonal Antibody, SSV Monoclonal Antibody, Improves Lung Injury and Survival in a Mouse Model of Lipopolysaccharide-Induced Sepsis-Like Syndrome. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055684
Modern Language Association (MLA)
Kusano, Toru…[et al.]. A Novel Anti-Histone H1 Monoclonal Antibody, SSV Monoclonal Antibody, Improves Lung Injury and Survival in a Mouse Model of Lipopolysaccharide-Induced Sepsis-Like Syndrome. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1055684
American Medical Association (AMA)
Kusano, Toru& Chiang, Kuei-Chen& Inomata, Masafumi& Shimada, Yayoi& Ohmori, Naoya& Goto, Takeshi…[et al.]. A Novel Anti-Histone H1 Monoclonal Antibody, SSV Monoclonal Antibody, Improves Lung Injury and Survival in a Mouse Model of Lipopolysaccharide-Induced Sepsis-Like Syndrome. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055684
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055684