Optimization of Fermentation Medium for Extracellular Lipase Production from Aspergillus niger Using Response Surface Methodology
Joint Authors
Lin, Carol Sze Ki
Jia, Jia
Yang, Xiaofeng
Wu, Zhiliang
Zhang, Qian
Lin, Zhi
Guo, Hongtao
Wang, Jianying
Wang, Yunshan
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-20
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Lipase produced by Aspergillus niger is widely used in various industries.
In this study, extracellular lipase production from an industrial producing strain of A.
niger was improved by medium optimization.
The secondary carbon source, nitrogen source, and lipid were found to be the three most influential factors for lipase production by single-factor experiments.
According to the statistical approach, the optimum values of three most influential parameters were determined: 10.5 g/L corn starch, 35.4 g/L soybean meal, and 10.9 g/L soybean oil.
Using this optimum medium, the best lipase activity was obtained at 2,171 U/mL, which was 16.4% higher than using the initial medium.
All these results confirmed the validity of the model.
Furthermore, results of the Box-Behnken Design and quadratic models analysis indicated that the carbon to nitrogen (C/N) ratio significantly influenced the enzyme production, which also suggested that more attention should be paid to the C/N ratio for the optimization of enzyme production.
American Psychological Association (APA)
Jia, Jia& Yang, Xiaofeng& Wu, Zhiliang& Zhang, Qian& Lin, Zhi& Guo, Hongtao…[et al.]. 2015. Optimization of Fermentation Medium for Extracellular Lipase Production from Aspergillus niger Using Response Surface Methodology. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055703
Modern Language Association (MLA)
Jia, Jia…[et al.]. Optimization of Fermentation Medium for Extracellular Lipase Production from Aspergillus niger Using Response Surface Methodology. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1055703
American Medical Association (AMA)
Jia, Jia& Yang, Xiaofeng& Wu, Zhiliang& Zhang, Qian& Lin, Zhi& Guo, Hongtao…[et al.]. Optimization of Fermentation Medium for Extracellular Lipase Production from Aspergillus niger Using Response Surface Methodology. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055703
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055703